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| 微波辅助PEG提取金银花叶中绿原酸的工艺优化 | |
| 引用本文： | 黄 荣,向 福,马文静,吴 伟,项 俊.微波辅助PEG提取金银花叶中绿原酸的工艺优化[J].中国酿造,2015,34(8):119. |
| 作者姓名： | 黄 荣 向 福 马文静 吴 伟 项 俊 |
| 作者单位： | 1.黄冈师范学院 生命科学学院，湖北黄州438000；2.大别山特色资源开发湖北省协同创新中心，湖北黄州438000； 3.湖北理工学院 医学院，湖北黄石435003 |
| 基金项目： | 湖北省教育厅科学研究计划重点项目（D20142902） |
| 摘 要： | 为了优化微波辅助聚二乙醇（PEG）提取金银花叶中绿原酸的工艺，通过单因素试验筛选提取温度、PEG-200体积分数、提取时间等关键影响因素，以绿原酸提取率为响应值设计响应面优化试验。结果表明：微波辅助PEG提取金银花叶中绿原酸的最佳工艺为微波功率350 W，微波时间70 s，料液比1∶20（g∶mL），提取温度82 ℃，PEG-200体积分数40%，提取时间24 min，该条件下绿原酸提取率为5.87%。该工艺简单可行、快速有效、绿色环保，可用于提取金银花叶中绿原酸工业化生产。 |
| 关 键 词： | 金银花叶 响应面 绿原酸 聚乙二醇 微波辅助提取 |
Optimization of microwave-assisted extraction of chlorogenic acid with PEG from Lonicerae japonica leaves |
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| HUANG Rong,XIANG Fu,MA Wenjing,WU Wei,XIANG Jun.Optimization of microwave-assisted extraction of chlorogenic acid with PEG from Lonicerae japonica leaves[J].China Brewing,2015,34(8):119. | |
| Authors: | HUANG Rong XIANG Fu MA Wenjing WU Wei XIANG Jun |
| Affiliation: | 1.College of Life Sciences, Huanggang Normal University, Huangzhou 438000, China; 2.Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huangzhou 438000, China; 3.Medical College, Hubei Polytechnic University, Huangshi 435003, China |
| Abstract: | To optimize the microwave-assisted extraction conditions of chlorogenic acid with PEG from Lonicerae japonica leaves, the key factors such as extraction time, temperature, and PEG-200 concentration were employed to design response surface analysis based on single factor tests with chlorogenic acid yield as response value. The optimum extraction conditions were microwave power 350 W, time 70 s, material-liquid ratio 1∶20 (g∶ml), extraction temperature 82 ℃, PEG-200 concentration 40%, and extraction time 24 min. Under the optimal process, the extraction yield of chlorogenic acid from L. japonica leaves reached 5.87%. The method was simple, green, rapid, effective, and suitable for industrialized production of chlorogenic acid from L. japonica leaves. |
| Keywords: | Lonicerae japonica leaves response surface method chlorogenic acid polyethylene glycol microwave-assisted extraction |
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