金氏真蛇尾多糖的抗氧化和抑菌活性研究

以黄海产金氏真蛇尾为原材料，采用碱提法，经脱脂、脱蛋白后得金氏真蛇尾多糖，通过Fenton反应和邻苯三酚自氧化法来测定多糖溶液对自由基的清除能力，采用滤纸片法测定金氏真蛇尾多糖对大肠杆菌、金黄色葡萄球菌、啤酒酵母菌和灰绿青霉的抑制作用。结果显示：金氏真蛇尾多糖对羟基自由基(·OH)和超氧阴离子(O2-·)具有较好的清除作用，并且清除率与多糖溶液呈现量效关系，多糖对羟基自由基(·OH)和超氧阴离子(O2-·)的最大清除率分别为45.68%和40.47%，表明蛇尾多糖具有一定的抗氧化活性，但是仍小于同质量浓度的VC溶液；金氏真蛇尾多糖对大肠杆菌、金黄色葡萄球菌和啤酒酵母的抑菌圈大小分别为13.46 mm、14.12 mm和14.73 mm，具有较好的抑菌作用，但对灰绿青霉则没有抑制作用。通过抗氧化和抑菌活性的研究，表明黄海产金氏真蛇尾多糖具有较好的抗氧化及抑菌活性。

Research on antioxidant and antibacterial activity of polysaccharide in Ophiura kinbergi

With Ophiura kinbergi in Huanghai Sea as raw materials, after degreasing and deproteinization, the polysaccharide in O. kinbergi was obtained by alkali extraction method. The capacity of scavenging free radicals of polysaccharide solution was measured by Fenton reaction and pyrogallol autoxidation method. The inhibiting effect of O. kinbergi polysaccharide on Escherichia coli, Staphylococcus aureus, Saccharomyces cerevisiae Hansen and Penicillium glaucum was measured by filtering paper method. The results showed that the O. kinbergi polysaccharide had good scavenging effect on hydroxyl radical (·OH) and superoxide anion radical (O2-·), and the clearance rate showed dose-effect relationship with polysaccharide solution. The maximum clearance rates of ·OH and O2-· of polysaccharide were 45.68% and 40.47%, respectively, which showed that the O. kinbergi polysaccharide had a certain antibacterial activity, but it was less than the antibacterial activity of VC in the same concentration. The O. kinbergi polysaccharide had good antibacterial activity on E. coli, S. aureus and S. cerevisiae Hansen. The diameters of antibacterial circle were 13.46 mm, 14.12 mm and 14.73 mm, but it had no antibacterial activity on P. glaucum. The study indicated the O. kinbergi polysaccharide had good antioxidant effect and antibacterial activity.