| 超声波辅助复合酶法提取枸杞多糖工艺研究 |
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| 引用本文： | 王杉杉,马韵升,姚 刚,刘圣鹏,虞凤慧,魏 征.超声波辅助复合酶法提取枸杞多糖工艺研究[J].中国酿造,2015,34(7):134. |
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| 作者姓名： | 王杉杉 马韵升 姚 刚 刘圣鹏 虞凤慧 魏 征 |
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| 作者单位： | 1.黄河三角洲京博化工研究院有限公司，山东滨州256500；2.山东京博控股股份有限公司，山东滨州256500 |
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| 基金项目： | 山东省科技发展计划项目（2013YD21001） |
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| 摘 要： | 以枸杞为试验材料，研究了超声波辅助复合酶（脂肪酶/蛋白酶/纤维素酶/果胶酶=1:1:1:1）提取枸杞多糖的工艺条件。以枸杞多糖得率为评价指标，通过正交试验确定了最佳提取条件为料液比1∶40（g∶mL），提取温度50 ℃，超声时间50 min，复合酶添加量0.5%。在此最佳条件下，枸杞多糖平均得率为58.910%。
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| 关 键 词： | 枸杞 多糖 超声波 复合酶 正交设计 |
Ultrasound-assisted compound enzyme extraction technology of polysaccharides from Lycium barbarum |
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| WANG Shanshan,MA Yunsheng,YAO Gang,LIU Shengpeng,YU Fenghui,WEI Zheng.Ultrasound-assisted compound enzyme extraction technology of polysaccharides from Lycium barbarum[J].China Brewing,2015,34(7):134. |
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| Authors: | WANG Shanshan MA Yunsheng YAO Gang LIU Shengpeng YU Fenghui WEI Zheng |
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| Affiliation: | 1.Yellow River Delta JingBo Research Institute of Chemical Industry Co., Ltd., Binzhou 256500, China;
2.Shandong Chambroad Holding Co., Ltd., Binzhou 256500, China |
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| Abstract: | The extraction condition of polysaccharide from Lycium barbarum was studied by ultrasound-assisted compound enzymes (lipase∶papain∶ cellulase∶pectinase =1∶1∶1∶1) extraction method. Using polysaccharide yield as evaluation index, the extraction condition was optimized by orthogonal experiment as follows: solid-liquid ratio 1∶40(g∶ml), extraction temperature 50 ℃, ultrasonic time 50 min and compound enzymes addition 0.5%. In this condition, the yield of L. barbarum polysaccharide was 58.910%. |
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| Keywords: | Lycium barbarum polysaccharids ultrasonic compound enzymes orthogonal experiments |
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