| 水酶法提取葡萄籽中蛋白质工艺的研究 |
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| 引用本文： | 尹 佳,王 璐,孙健英,蔡莹莹,隋 新. 水酶法提取葡萄籽中蛋白质工艺的研究[J]. 中国酿造, 2015, 34(12): 122. DOI: 10.11882/j.issn.0254-5071.2015.12.027 |
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| 作者姓名： | 尹 佳 王 璐 孙健英 蔡莹莹 隋 新 |
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| 作者单位： | 吉林化工学院生物与食品工程学院，吉林吉林132022 |
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| 基金项目： | 吉林省大学生创新创业训练计划项目（吉教高字[2015]17号） |
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| 摘 要： | 采用木瓜蛋白酶对脱脂葡萄籽进行酶解提取蛋白质。在单因素试验基础上，考察酶用量、酶解温度、pH值、反应时间和料液比对葡萄籽蛋白质提取率的影响，采用响应面法对葡萄籽蛋白质水酶法提取条件进行了优化。结果表明，葡萄籽蛋白质的最优提取条件为：酶解温度40 ℃，料液比1∶25（g∶mL），酶用量4%，酶解时间45 min。在此优化条件下，葡萄籽蛋白质提取率为67.85%。
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| 关 键 词： | 水酶法 葡萄籽蛋白质 响应面法 提取条件 |
Extraction technology of protein from grape seeds by aqueous enzymatic method |
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| YIN Jia,WANG Lu,SUN Jianying,CAI Yingying,SUI Xin. Extraction technology of protein from grape seeds by aqueous enzymatic method[J]. China Brewing, 2015, 34(12): 122. DOI: 10.11882/j.issn.0254-5071.2015.12.027 |
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| Authors: | YIN Jia WANG Lu SUN Jianying CAI Yingying SUI Xin |
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| Affiliation: | College of Biology and Food Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China |
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| Abstract: | Protein was extracted from defatted grape seeds by hydrolysis of papain. Base on single factor experiments, the effect of enzyme addition, enzymolysis temperature, pH, time and solid-liquid ratio on grape seed protein extraction rate was investigated. Aqueous enzymatic method extraction conditions of grape seeds protein were optimized by response surface methodology. Results indicated that the optimum extraction conditions were as follows: enzymolysis temperature 40 ℃, solid-liquid ratio 1∶25 (g∶ml), enzyme addition 4% and enzymolysis time 45 min. Under this condition, the grape seed protein extraction rate was 67.85%. |
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| Keywords: | aqueous enzymatic method grape seeds protein response surface methodology extraction conditions |
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