|
|||||
|
|
| 不同生化因子对米曲霉蛋白酶系活力的影响 | |
| 引用本文： | 刘 源,李诗雯,彭铭烨,曹约泽,付彩霞,高 冰,汪 超,李冬生,徐 宁.不同生化因子对米曲霉蛋白酶系活力的影响[J].中国酿造,2015,34(12):55. |
| 作者姓名： | 刘 源 李诗雯 彭铭烨 曹约泽 付彩霞 高 冰 汪 超 李冬生 徐 宁 |
| 作者单位： | 1.湖北工业大学生物工程学院，工业发酵湖北省协同创新中心湖北省食品发酵工程技术研究中心，湖北武汉430068； 2.湖北顺溪生物食品股份有限公司，湖北十堰442300；3.湖北土老憨调味食品股份有限公司，湖北宜昌443000 |
| 基金项目： | 国家级大学生创新创业训练计划项目（20130500009）；湖北省教育厅项目（Q20141409）；湖北工业大学博士科研启动基金（BSQD12147） |
| 摘 要： | 研究不同生化因子（金属离子、乙二胺四乙酸（EDTA）和十二烷基磺酸钠（SDS））对米曲霉蛋白酶系（包括酸性蛋白酶、中性蛋白酶、碱性蛋白酶、氨肽酶和羧肽酶等5种）活力的影响，结果显示：生化因子都对羧肽酶活力有促进作用，影响最强的是Ca2+，其相对酶活达到310%；对酸性蛋白酶活力促进作用最强的是的Mn2+，相对酶活达到317%；而SDS完全抑制其活性。生化因子对中性蛋白酶、碱性蛋白酶和氨肽酶活力主要起抑制作用，抑制能力最强的分别是Ge2+，Fe3+和SDS。Ag+对氨肽酶活力促进显著，相对酶活力达到483%。 |
| 关 键 词： | 米曲霉 蛋白酶 生化因子 酶活力 |
Effect of different biochemical reagents on proteases activity of Aspergillus oryzae |
|
| LIU Yuan,LI Shiwen,PENG Mingye,CAO Yueze,FU Caixia,GAO Bing,WANG Chao,LI Dongsheng,XU Ning.Effect of different biochemical reagents on proteases activity of Aspergillus oryzae[J].China Brewing,2015,34(12):55. | |
| Authors: | LIU Yuan LI Shiwen PENG Mingye CAO Yueze FU Caixia GAO Bing WANG Chao LI Dongsheng XU Ning |
| Affiliation: | 1.Hubei Research Center of Food Fermentation Engineering and Technology, Hubei Collaborative Innovation Center for Industrial Fermentation, College of Biological Engineering, Hubei University of Technology, Wuhan 430068, China; 2. Hubei Shunxi Biological Food Co., Ltd., Shiyan 442300, China; 3.Hubei Tulaohan Flavouring and Food Co., Ltd., Yichang 443000, China |
| Abstract: | The effect of different biochemical reagents(metal ions, EDTA, and SDS) on proteases (acid protease, neutral protease, alkaline protease, aminopeptidase and carboxypeptidase) activity of Aspergillus oryzae was researched. The results showed that the all biochemical reagents could improve the carboxypeptidase activity, Ca2+ had the greatest effect on carboxypeptidase activity and the relative enzyme activity reached 310%. Mn2+ had greatest effect on acid protease activity, and the relative enzyme activity reached 317%. The acid protease activity was completely inhibited by SDS. Biochemical reagents inhibited the activity of neutral protease, alkaline protease and aminopeptidase. The biochemical reagents with greatest inhibition in order were Ge2+, Fe3+ and SDS, respectively. Ag+ had significantly promoting effect on aminopeptidase activity, and the relative enzyme activity reached 483%. |
| Keywords: | Aspergillus oryzae protease biochemical reagent enzyme activity |
| 本文献已被 CNKI 等数据库收录！ | |
| 点击此处可从《中国酿造》浏览原始摘要信息 | |
| 点击此处可从《中国酿造》下载全文 | |