草鱼鱼肠抗菌活性物质的提取

为优化草鱼鱼肠抗菌活性物质的提取条件，以草鱼鱼肠为原料，通过单因素试验和正交试验研究乙酸体积分数、料液比和超声时间对提取草鱼肠道抗菌物质的影响。试验结果表明，影响草鱼肠道抗菌物质提取的主次因素依次为乙酸体积分数＞料液比＞超声时间；草鱼肠道抗菌物质的最佳提取条件为：乙酸体积分数10%，料液比1∶3.5（g∶mL），超声时间10 min，4 ℃浸提24 h。在此最佳提取条件下，提取液的抗菌活性较高，金黄色葡萄球菌抑菌圈直径为3.106 0 cm，大肠杆菌抑菌圈直径为2.028 4 cm。

Extraction of grass carp intestine antimicrobial substances

In order to optimize the extraction conditions of grass carp intestinal antimicrobial substances, using grass carp intestine as raw material, the influence of acetic acid concentration, material to liquor ratio and ultrasonic time on preliminary extraction of grass carp intestinal antimicrobial substances was determined by single factor and orthogonal experiment. The test results showed that factors influencing the grass carp intestinal antimicrobial substances extraction in order were acetic acid concentration, material to liquor ratio, ultrasonic time. The optimal preliminary extracting condition were as follows: acetic acid concentration 10%, material to liquor ratio 1∶3.5 (g∶ml), ultrasonic time 10 min, socking 24 h at 4 ℃. Under this condition, the antimicrobial activity was effective, the antibacterial circle diameter of Staphylococcus aureus was 3.106 0 cm, and the antibacterial circle diameter of Escherichia coli was 2.028 4 cm.