| 产纤溶酶海洋放线菌的筛选及初步鉴定 |
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| 引用本文： | 董 超,米 阳,原晋波,史延茂.产纤溶酶海洋放线菌的筛选及初步鉴定[J].中国酿造,2015,34(7):59. |
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| 作者姓名： | 董 超 米 阳 原晋波 史延茂 |
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| 作者单位： | 1.河北省科学院生物研究所，河北石家庄050081；2.河北工业大学化工学院，天津300130 |
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| 基金项目： | 2014年河北省科技厅科技支撑项目（14273201D） |
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| 摘 要： | 从渤海湾海水和海泥样本中筛选得到产纤溶酶的菌株。通过添加抑制剂对样品预处理、调整培养基配方和添加目标蛋白诱导培养以筛选得到菌株，通过生理生化实验和16S rRNA序列分析，进行菌株种属鉴定。结果表明，调整富集和筛选的培养基配方，获得了一株产纤溶酶的菌株MY0504，初步推断为链霉菌属（Streptomyces sp.）。
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| 关 键 词： | 海洋微生物 纤溶酶 放线菌 |
Screening and preliminary identification of a fibrinolytic enzyme-producing marine actinomycete strain |
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| DONG Chao,MI Yang,YUAN Jinbo,SHI Yanmao.Screening and preliminary identification of a fibrinolytic enzyme-producing marine actinomycete strain[J].China Brewing,2015,34(7):59. |
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| Authors: | DONG Chao MI Yang YUAN Jinbo SHI Yanmao |
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| Affiliation: | 1.Institute of Biology, Hebei Academy of Science, Shijiazhuang 050081, China;
2.College of Chemistry and Chemical Engineering, Hebei University of Technology, Tianjin 300130, China |
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| Abstract: | A fibrinolytic enzyme-producing strain was screened from Bohai Bay seawater and sea mud sample. The sample strains were pretreated by adding inhibitors, adjusting medium formula, and inducing culture by adding target protein to screen the target strain. The strain was identified by physiological and biochemical experiments and 16S rDNA sequence analysis. The results showed that after adjusting the medium formula, strains with big differences can be obtained. A strain MY0504 which can produce fibrinolytic enzyme was isolated and identified as Streptomyces sp. |
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| Keywords: | marine microorganisms fibrinolytic enzyme actinomycetes |
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