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| 金银花中绿原酸的双水相萃取及抗氧化能力研究 | |
| 引用本文： | 胡佳钦,向 福,石长萍,吴 伟,方元平. 金银花中绿原酸的双水相萃取及抗氧化能力研究[J]. 中国酿造, 2015, 34(12): 109. DOI: 10.11882/j.issn.0254-5071.2015.12.024 |
| 作者姓名： | 胡佳钦 向 福 石长萍 吴 伟 方元平 |
| 作者单位： | 1.黄冈师范学院生命科学学院，湖北黄州438000；2.大别山特色资源开发湖北省协同创新中心，湖北黄州438000；3.湖北理工学院医学院，湖北黄石435003 |
| 基金项目： | 湖北省自然科学基金重点项目（2014CFA129） |
| 摘 要： | 为合理利用大别山金银花资源，以罗田县的金银花及其茎、叶为试验材料，利用双水相体系萃取绿原酸，并研究了其抗氧化活性。结果表明：50 mL丙酮/磷酸氢二钾(K2HPO4)双水相体系萃取绿原酸的最佳条件为酮水比8.5∶1.5(V/V)，磷酸氢二钾添加量3 g，水提液绿原酸质量浓度22.9 g/L。在此条件下，绿原酸含量为56.27%，收率为98.46%，且其绿原酸的羟基自由基清除能力较强，从而为大别山金银花资源的开发利用提供了理论依据。 |
| 关 键 词： | 双水相 金银花 绿原酸 羟基自由基清除 抗氧化活性 |
Aqueous two-phase extraction and antioxidant activity of chlorogenic acid in honeysuckle |
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| HU Jiaqin,XIANG Fu,SHI Changping,WU Wei,FANG Yuanping. Aqueous two-phase extraction and antioxidant activity of chlorogenic acid in honeysuckle[J]. China Brewing, 2015, 34(12): 109. DOI: 10.11882/j.issn.0254-5071.2015.12.024 | |
| Authors: | HU Jiaqin XIANG Fu SHI Changping WU Wei FANG Yuanping |
| Affiliation: | 1.College of Life Sciences, Huanggang Normal University, Huangzhou 438000, China; 2.Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huangzhou 438000, China; 3.School of Medicine, Hubei Polytechnic University, Huangshi 435003, China |
| Abstract: | For reasonable utilization of Dabie Mountains honeysuckle resources, using the flowers, branches and leaves of honeysuckle as test materials, the chlorogenic acid was extracted by aqueous two-phase system, and the antioxidant activity was researched. Results indicated that the optimum extraction conditions of chlorogenic acid in 50 ml acetone and K2HPO4 aqueous two-phase system were ketone-water ratio 8.5∶1.5(V/V), K2HPO4 addition 3 g, water extract chlorogenic acid content 22.9 g/L. Under the conditions, the content and yield of chlorogenic acid was 56.27% and 98.4%, respectively. The chlorogenic acid had a good capacity of scavenging hydroxyl radical, which provided a theoretical basis for the development and application of Dabie Mountains honeysuckle resources. |
| Keywords: | aqueous two-phase honeysuckle chlorogenic acid hydroxyl radical scavenging antioxidant activity |
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