| 铜绿假单胞菌对萘和菲降解特性研究 |
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| 引用本文： | 汪如婷,雒晓芳,田丹妮,莫芳芳,马麟龙. 铜绿假单胞菌对萘和菲降解特性研究[J]. 中国酿造, 2015, 34(10): 82. DOI: 10.11882/j.issn.0254-5071.2015.10.018 |
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| 作者姓名： | 汪如婷 雒晓芳 田丹妮 莫芳芳 马麟龙 |
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| 作者单位： | 1.西北民族大学生命科学与工程学院，甘肃兰州730100；2.西北民族大学实验中心，甘肃兰州730100 |
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| 基金项目： | 西北民族大学2015年大学生创新创业训练计划项目（201510742079）；西北民族大学2015年本科生科研创新项目（URIP15166） |
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| 摘 要： | 研究不同pH和温度条件下，铜绿假单胞菌（Pseudomonas aeruginosa）对多环芳烃中萘和菲的降解效果。结果表明，以活性炭为吸附载体，多环芳烃为唯一碳源，将此菌株培养48 h后，pH值为7.0时，萘的降解率可达25.68%，脱氢酶活性达到33.42 （μg/mL）·h；菲的降解率可达41.9%，脱氢酶活性达到37.44 （μg/mL）·h。当温度为37 ℃时，萘的降解率可达27.7%，脱氢酶活性达到33.60 （μg/mL）·h；菲的降解率可达34.5%，脱氢酶活性达到39.96 （μg/mL）·h。
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| 关 键 词： | 铜绿假单胞菌 萘 菲 降解率 脱氢酶活性 |
Research on the degradation characteristics of naphthalene and phenanthrene by Pseudomonas aeruginosa |
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| WANG Ruting,LUO Xiaofang,TIAN Danni,MO Fangfang,MA Linlong. Research on the degradation characteristics of naphthalene and phenanthrene by Pseudomonas aeruginosa[J]. China Brewing, 2015, 34(10): 82. DOI: 10.11882/j.issn.0254-5071.2015.10.018 |
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| Authors: | WANG Ruting LUO Xiaofang TIAN Danni MO Fangfang MA Linlong |
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| Affiliation: | 1.Life Science and Engineering, Northwest University for Nationalities, Lanzhou 730100, China;
2.Center of Experiment, Northwest University for Nationalities, Lanzhou 730100, China |
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| Abstract: | The effect of Pseudomonas aeruginosa on naphthalene and phenanthrene degradation was studied under the condition of different pH and temperature. The results showed that using activated carbon as carrier, polycyclic aromatic hydrocarbons as sole carbon source, when the strain was cultured for 48 h at pH 7, the degradation rate of naphthalene was 25.68%, the dehydrogenase activity reached 33.42 (μg/ml)·h, the degradation rate of phenanthrene reached 41.9%, and the dehydrogenase activity reached 37.44 (μg/ml)·h. When the temperature was 37 ℃, the degradation rate of naphthalene was 27.7%, the dehydrogenase activity reached 33.60 (μg/ml)·h, the degradation rate of phenanthrene was 34.5%, and the dehydrogenase activity reached 39.96 (μg/ml)·h. |
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| Keywords: | Pseudomonas aeruginosa naphthalene phenanthrene degradation rate dehydrogenase activity |
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