酸酶法降解稻草纤维素工艺优化及其发酵产乙醇的研究

对纤维素降解工艺进行了优化，并将其应用于淀粉质原料酿酒中，在减少大米用料的同时增加发酵液糖含量，提高酒度，节约生产成本。实验采用酸法酶法两步处理稻草秸秆纤维素，结果表明，在盐酸浓度2．5％，温度为126℃，固液比（g/mL）为1：2的条件下处理1h，再在温度为50℃，pH值为5．0，酶用量为35IU／g干物质（自制酶液）的条件下水解12h，最终葡萄糖得率为24．5％。水解糖液与大米糖化液按6：4（v／v）配比混合，接入酿酒酵母发酵，产酒蒸馏酒度为13．4％vol。

Study on Technical Optimization of the Hydrolysis of Straw Cellulose by Acid-enzymatic Method and Its Fermentation to Produce Ethanol

The hydrolysis of straw cellulose was optimized and then applied in liquor-making by starch raw materials which could reduce the use level of rice,increase sugar content in fermenting liquid,increase liquor alcoholicity,and save production cost.In the experiments,straw cellulose was pretreated by acid method and enzymatic method as follows: straw stalk treated for 1h in conditions of hydrochloric acid concentration of 2.5 % and temperature at 126 ℃ and the ratio of solids to liquid(g/mL) as 1∶2,then 12 h hydrolysis in conditions of temperature at 50 ℃,pH=5.0,and enzyme use level/dry substance=35IU/g(self-made enzyme liquid used).As a result,final glucose yield was 24.5 %.The hydrolyzed sugar liquid mixed with rice saccharifying liquid according to the ratio of 6∶4(v/v) and then fermented by yeast,and the alcoholicity of the distilled liquor was as high as 13.4 %vol.