| 高效液相-蒸发光散射检测器(HPLC-ELSD)测定食品中三氯蔗糖的方法研究 |
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| 引用本文： | 梁桂娟,冯永渝,黄永光. 高效液相-蒸发光散射检测器(HPLC-ELSD)测定食品中三氯蔗糖的方法研究[J]. 酿酒科技, 2012, 0(5): 104-106,109 |
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| 作者姓名： | 梁桂娟 冯永渝 黄永光 |
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| 作者单位： | 1. 贵州省产品质量监督检验院,贵州贵阳,550004
2. 贵州省轻工业科学研究所．贵州贵阳550007 |
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| 摘 要： | 建立用高效液相-蒸发光散射检测器（HPLC—ELSD）快速准确测定酱油、配制酒等食品中三氯蔗糖的方法。样品经前处理后，用HPLC—ELSD进行检测。仪器条件为流动相乙腈／水为20／80，流速为1．0mL／min，雾化气流量为2．9L／min，漂移管温度为106℃。结果表明，该方法在0-100wedmL范围内曲线方程的相关系数R2为0．9996，方法检出限为0．5mg／kg，样品回收率大于90％，RSD小于5％。该方法操作简便、稳定可靠，适用于配制酒等食品中三氯蔗糖的检测。
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| 关 键 词： | 三氯蔗糖 高效液相色谱 蒸发光散射检测器 |
Investigation on the Measurement of Sucralose in Food by HPLC-ELSD |
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| LIANG Guijuan,FENG Yongyu,and HUANG Yongguang. Investigation on the Measurement of Sucralose in Food by HPLC-ELSD[J]. Liquor-making Science & Technology, 2012, 0(5): 104-106,109 |
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| Authors: | LIANG Guijuan FENG Yongyu and HUANG Yongguang |
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| Affiliation: | 1.Guizhou Provincial Product Quality Supervision and Determination Institute,Guiyang,Guizhou 550004; 2.Guizhou Provincial Light Industry Scientific Research Institute,Guiyang,Guizhou 550007,China) |
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| Abstract: | A method of HPLC-ELSD for fast and accurate measurement of sucralose in food had been developed.Food sample was firstly pretreated and then determined by HPLC-ELSD.The mobile phase was made of 20 % acetonitrile and 80 % water with flow rate as 1.0 mL/min.The temperature of drift tube was at 106 ℃ and the rate of flow of atomized gas was 2.9 L/min.The measurement results indicated that the range of the calibration curve of HPLC-ELSD were from 0～100 μg/mL(r=0.99993),the recoveries were above 90 %,and the relative standard deviations were lower than 5 %,and the detection limit was 0.5 mg/kg.Such method was accurate,reliable and fast,which could be used to measure sucralose in food. |
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| Keywords: | sucralose high performance liquid chromatography evaporative light scattering detectors |
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