| 高效丢糟纤维素分解菌的筛选及产酶研究 |
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| 引用本文： | 程驰,周健,罗惠波,杨欣怡,刘小俊,周祥.高效丢糟纤维素分解菌的筛选及产酶研究[J].酿酒科技,2012(6):96-98. |
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| 作者姓名： | 程驰 周健 罗惠波 杨欣怡 刘小俊 周祥 |
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| 作者单位： | 1. 四川理工学院生物工程学院,四川自贡643000 生物资源与生态环境教育部重点实验室四川大学生命科学学院,四川成都610064
2. 四川理工学院生物工程学院,四川自贡,643000 |
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| 基金项目： | 酿酒生物技术及应用四川省重点实验室开放基金项目，四川省教育厅项目，自贡市科技局重点科技计划项目，四川理工学院“大学生创新基金重点项目” |
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| 摘 要： | 从白酒丢糟堆腐物中筛选出5株分解丢糟纤维素能力强的菌株,以其中降解能力最强的绿色木霉S7为出发菌株。通过对其固态发酵产酶条件的单因素优化试验,初步确定其固体培养基质中丢糟粉与麸皮的最适质量比为4∶1,硫酸铵2%,初始pH值为4.0,30～35℃发酵96 h,羧甲基纤维素酶活和滤纸酶活分别达到57.08μg/min.mL和26.02μg/min.mL。
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| 关 键 词： | 白酒丢糟 纤维素酶 绿色木霉 固态发酵 |
Screening of A High-efficiency Cellulose-decomposing Bacteria Strain and Study on Its Cellulase-producing Conditions |
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| CHENG Chi,ZHOU Jian,LUO Huibo,YANG Xinyi,LIU Xiaojun,and ZHOU Xiang.Screening of A High-efficiency Cellulose-decomposing Bacteria Strain and Study on Its Cellulase-producing Conditions[J].Liquor-making Science & Technology,2012(6):96-98. |
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| Authors: | CHENG Chi ZHOU Jian LUO Huibo YANG Xinyi LIU Xiaojun and ZHOU Xiang |
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| Affiliation: | 1(1.Department of Biology Engineering,Sichuan University of Science & Engineering,Zigong,Sichuan 643000;2.Key Lab for Resource and Biological Ecological Environment of Education Ministry,College of Life Science,Sichuan University,Chengdu,Sichuan 610064,China) |
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| Abstract: | 5 high-efficiency cellulose-decomposing bacteria strains were isolated from liquor spent grains,among them,Trichoderma viride strain S7 with the strongest decomposing capability was used as the starting strain for single factor optimization test of its cellulase-production conditions by solid fermentation,and the optimized conditions were summed up as follows: the optimum mass ratio of spent grains powder and wheat bran was 4∶1 in solid culture mediums,ammonium sulphate content was 2 %,initial pH value was 4.0,and 96 h fermentation at 30～35 ℃,finally,the activities of carboxymethyl cellulase and filter paper enzyme could reach up to 57.08 μg/minomL and 26.02 μg/minomL respectively. |
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| Keywords: | spent grains cellulase Trichoderma viride solid fermentation |
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