| 进口葡萄酒中赭曲霉毒素A的含量分析 |
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| 引用本文： | 陈杰,莫艳霞,徐银君,倪梅林,陈树兵. 进口葡萄酒中赭曲霉毒素A的含量分析[J]. 酿酒科技, 2012, 0(1): 107-109 |
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| 作者姓名： | 陈杰 莫艳霞 徐银君 倪梅林 陈树兵 |
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| 作者单位： | 宁波出入境检验检疫局,浙江宁波,315000 |
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| 基金项目： | 国家质检总局科技计划项目 |
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| 摘 要： | 为了解进口葡萄酒中赭曲霉毒素A的含量和污染状况,本研究建立了无需净化、浓缩,直接进样,采用液相色谱-串联质谱联用法(LC-MS/MS)测定葡萄酒中赭曲霉毒素A的方法。赭曲霉毒素A空白基质标准溶液在1.0～20.0 ng/mL浓度范围内线性良好,方法检测限达到0.05 ng/mL,添加浓度为2.0 ng/mL质控样品的回收率为98.7%～113.2%,精密度<5.7%。应用该方法测定了84个进口葡萄酒样品中赭曲霉毒素A的含量,结果表明,赭曲霉毒素的检出率为100%,含量为0.14～1.10 ng/mL,平均含量为0.32 ng/mL。
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| 关 键 词： | 进口葡萄酒 赭曲霉毒素A 含量状况 |
Analysis of Ochratoxin A Content in Imported Grape Wines |
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| Affiliation: | CHEN Jie,MO Yanxia,XU Yinjun,NI meilin and CHEN Shubin(Ningbo Exit & Entry Inspection and Quarantine Bureau,Ningbo,Zhejiang 315000,China) |
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| Abstract: | To learn about Ochratoxin A(OTA) content in imported grape wines,in this study,a new LC-MS/MS method for the detection of OTA content in grape wine was proposed,by directly injecting the sample in chromatographic system without any clean-up or concentration.The blank matrix standard calibration curve was linear in the concentration range of 1.0～20.0 ng/mL,the correlation coefficient was 0.9999,the detection limit was 0.05 ng/mL,and the recoveries ranged from 98.7 % to 113.2 % with RSD of 5.7 % for 5 spiked wine samples at 2.0 ng/mL.84 grape wine samples from different countries or areas were analyzed with this method.As a result,all the samples were contaminated by OTA with the concentration from 0.14 to 1.10 ng/mL and the average concentration was 0.32 ng/mL. |
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| Keywords: | imported grape wine Ochratoxin A status |
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