| 核茎点霉发酵产纤维素酶发酵条件优化 |
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| 引用本文： | 潘乐毅,殷江南,田云,卢向阳. 核茎点霉发酵产纤维素酶发酵条件优化[J]. 酿酒科技, 2012, 0(5): 21-23 |
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| 作者姓名： | 潘乐毅 殷江南 田云 卢向阳 |
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| 作者单位： | 1. 湖南省农业生物工程研究所,湖南长沙410128 湖南农业大学生物科学技术学院,湖南长沙410128
2. 湖南省农业生物工程研究所,湖南长沙,410128 |
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| 基金项目： | 国家“十一五”科技支撑计划，科技部国际科技合作项目，长江学者和创新团队发展计划，湖南省研究生科研创新项目 |
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| 摘 要： | 前期的单因素试验研究表明，在核茎点霉（Phoma tputaminum）LYYZ90-19发酵产纤维素酶过程中，温度、pH值及接种量是影响产酶量的关键因素。在此基础上，运用响应面分析法，以酶活力值为响应值，建立酶活力值与温度、prI值及接种量这3个关键因子的二次多项式回归模型，以Design Expert软件对模型进行分析并优化。研究表明，核茎点霉发酵产纤维素酶最佳工艺条件为：温度27．4℃、pH6．88、接种量6．75％。在该工业条件下，发酵液酶活为（18．21±0-3）U／mL。
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| 关 键 词： | 微生物 纤维素酶 发酵 响应面法 |
Optimization of Fermenting Conditions of Phoma putaminum LYYZ90-19 for Cellulase Production by Response Surface Design |
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| PAN Leyi,YIN Jiangnan,TIAN Yun,and LU Xiangyang. Optimization of Fermenting Conditions of Phoma putaminum LYYZ90-19 for Cellulase Production by Response Surface Design[J]. Liquor-making Science & Technology, 2012, 0(5): 21-23 |
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| Authors: | PAN Leyi YIN Jiangnan TIAN Yun and LU Xiangyang |
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| Affiliation: | 1,2(1.Hunan Agricultural Bioengineering Research Institute,Changsha,Hu’nan 410128;2.College of Bioscience and Biotechnology, Hunan Agricultural University,Changsha,Hu’nan 410128,China) |
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| Abstract: | Previous single factor test results indicated that temperature,pH value and inoculating quantity were the key factors influencing cellulase yield of Phoma putaminum LYYZ90-19.Based on previous study,response surface method was applied to construct two degree polynomial regression models of the three key factors with cellulase activity as response values,and Design Expert software was used to optimize these models.The results showed that the optimal conditions were as follows: temperature,pH and inoculating quantity were 27.4 ℃,6.88 and 6.75 % respectively.Under the optimum conditions,the produced cellulase activity was as high as(18.21±0.3) U/mL. |
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| Keywords: | microbe cellulase fermentation response surface design |
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