| 液相色谱法测定白酒中安赛蜜和糖精钠含量 |
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| 引用本文： | 程春梅,赵燕,师邱毅,张境,宋慧波.液相色谱法测定白酒中安赛蜜和糖精钠含量[J].酿酒科技,2012(6):99-101. |
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| 作者姓名： | 程春梅 赵燕 师邱毅 张境 宋慧波 |
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| 作者单位： | 1. 浙江医药高等专科学校,浙江宁波,315100
2. 淮安市产品质量监督检验所,江苏淮安,223001 |
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| 基金项目： | 浙江医药高等专科学校校级科研课题 |
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| 摘 要： | 建立了高效液相色谱测定白酒中安赛蜜、糖精钠的方法。采用在沸水浴条件下,将白酒样品50.0 g用旋转蒸发仪减压蒸发至近干后用水定容至10 mL,进液相色谱分析;用AQ-C18色谱柱（250×4.6 mm）,以0.02 mol/L的乙酸铵-甲醇（90∶10）为流动相,二极管阵列检测器在波长220 nm进行检测。结果表明,该方法的检出限低至安赛蜜0.014 mg/kg,糖精钠0.019 mg/kg,被测样品加标回收率：安赛蜜为97.5%,糖精钠102.0%。
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| 关 键 词： | 高效液相色谱法 白酒 安赛蜜 糖精钠 |
Determination of Acesulfame-K and Saccharin Sodium Content in Liquor by High Performance Liquid Chromatography |
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| CHENG Chunmei,ZHAO Yan,SHI Qiuyi,ZHANG Jing,and SONG Huibo.Determination of Acesulfame-K and Saccharin Sodium Content in Liquor by High Performance Liquid Chromatography[J].Liquor-making Science & Technology,2012(6):99-101. |
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| Authors: | CHENG Chunmei ZHAO Yan SHI Qiuyi ZHANG Jing and SONG Huibo |
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| Affiliation: | 1(1.Zhejiang Pharmaceutical College,Ningbo,Zhejiang 315100;2.Huai’an Institute of Supervision and Inspection on Product Quality,Huai’an,Jiangsu 223001,China) |
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| Abstract: | A HPLC method for simultaneous separation and determination of acesulfame-K and saccharin sodium in liquor had been developed.Under boiling water bath conditions,vacuum evaporation of 50.0 g liquor samples was carried out to almost dry by rotary evaporation instrument and then constant volume to 10 mL by use of water for HPLC analysis,an AQ-C18 column(250×4.6 mm) was used with 0.02 mol/L NH4Ac-methanol(90∶10) as eluent,then detected at 230 nm through a photo-diode array detector.The detection limits of acesulfame-K and saccharin sodium were 0.014 mg/kg and 0.019mg/kg respectively,and their recovery rate were 97.5 and 102.0 % respectively. |
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| Keywords: | HPLC liquor acesulfame-K saccharin sodium |
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