Characterization of Fish Myofibrillar Protein by Conjugation with Alginate Oligosaccharide Prepared Using Genetic Recombinant Alginate Lyase |
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| Authors: | Ryo Sato Tomoo Sawabe Hiroki Saeki |
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| Affiliation: | Authors are with Graduate School of Fisheries Sciences, Hokkaido Univ., Minato 3, Hakodate, Hokkaido 041–8611, Japan. Direct inquiries to author Saeki (E-mail: ). |
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| Abstract: | ABSTRACT: The production of alginate lyase using genetically modified Escherichia coli was superior to the purification of alginate lyase from a culture medium of Pseudoalteromonas elyakovii regarding production efficiency. When alginate oligosaccharide (AO) prepared using genetic recombinant alginate lyase was introduced to fish myofibrillar proteins, the protein obtained high water solubility and improved thermal stability, similarly to AO prepared using wild-type lyase. Therefore, the use of genetic recombinant technology for the production of alginate lyase would be useful for the functional improvement of fish myofibrillar proteins by conjugation with AO. |
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| Keywords: | fish protein alginate oligosaccharide genetic recombinant alginate lyase solubility thermal stability |
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