| 絮凝剂产生菌KJ-10原生质体的制备及再生条件研究 |
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| 引用本文： | 赵东风,顾贵洲,李刚,张云波,刘其友. 絮凝剂产生菌KJ-10原生质体的制备及再生条件研究[J]. 化学与生物工程, 2011, 28(3): 24-26,52. DOI: 10.3969/j.issn.1672-5425.2011.03.007 |
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| 作者姓名： | 赵东风 顾贵洲 李刚 张云波 刘其友 |
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| 作者单位： | 1. 中国石油大学化学化工学院,山东,青岛,266555
2. 新疆克拉玛依市环境科研监测中心站,新疆,克拉玛依,834000 |
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| 基金项目： | 中国石油天然气股份有限公司科学研究与技术开发项目 |
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| 摘 要： | 从活性污泥中筛选出具备较高絮凝活性的菌株KJ-10.对菌株KJ-10原生质体的制备和再生的影响因素进行了考察.结果表明,KJ-10原生质体制备和再生的优化务件为:以1%接种量在CM液体培养基中活化12 h,酶解前加入5 mol·L<'-1>甘氨酸预处理12 h,酶浓度为0.1 mg·mL<'-1>,37℃水浴酶解120...
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| 关 键 词： | 原生质体制备 原生质体再生 絮凝剂产生菌 |
Study on Production and Regeneration of Flocculant-Producing Bacterium KJ-10 Protoplast |
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| ZHAO Dong-feng,GU Gui-zhou,LI Gang,ZHANG Yun-bo,LIU Qi-you. Study on Production and Regeneration of Flocculant-Producing Bacterium KJ-10 Protoplast[J]. Chemistry & Bioengineering, 2011, 28(3): 24-26,52. DOI: 10.3969/j.issn.1672-5425.2011.03.007 |
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| Authors: | ZHAO Dong-feng GU Gui-zhou LI Gang ZHANG Yun-bo LIU Qi-you |
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| Affiliation: | 1(1.College of Chemistry and Chemical Engineering,China University of Petroleum,Qingdao 266555,China;2.Xinjiang Karamay Environmental Monitor Center,Karamay 834000,China) |
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| Abstract: | The strain KJ-10 screened from the activated sludge had high flocculation activity.The influential factors for production and regeneration of flocculant-producing bacterium KJ-10 protoplast were investigated.The optimum conditions for production and regeneration of KJ-10 protoplast were determined as follows: activation for 12 h with 1% inoculation volume of liquid medium CM;pretreating with 5 mol·L-1 glycine for 12 h before enzymatic hydrolysis;enzymatic hydrolysis for 120 min in 37℃ water bath with enzyme concentration 0.1 mg·mL-1;coating on a renewable flat with 0.5 mol·L-1 of sucrose as stabilizer.The formation rate and regeneration rate of protoplast reached 93.5%,20.5%,respectively. |
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| Keywords: | protoplast production protoplast regeneration flocculant-producing bacterium |
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