空间细胞培养装置设计与流体动力学仿真优化

细胞培养装置可用于细胞生物学、空间生物技术和航天医学的研究。设计并制作了一种流加式细胞自动培养装置，可适用于空间、地面微重力环境下的细胞体外自动培养，定期给细胞培养板提供不同试剂定时定量的输入，满足空间细胞培养所要求的小型化、方便组装和高可靠性的要求。整体装置采用模块化的设计方法，利用Solid Works软件分别构建基础组件、试剂供应组件和培养组件三维模型，并使用Solid Works软件中Flow Simulation模块对细胞培养装置与细胞培养板连接的关键部件—针板，进行有限元分析和流体仿真结构优化，观察其内部通道的速度场和压力场，采用圆角替换直角的方法优化通道。仿真结果表明，该装置切实可行，能满足细胞培养装置所需条件，优化后的针板通道内流体稳定性更强，流速及压力分布更均匀。在地面环境中，优化后的针板在低、中、高流速下，流体在横向通道内的速度均匀度分别提升了95%、96%、94%,在纵向通道内的速度均匀度分别提升了68%、70%、72%。实验结果表明，流体在优化后针板通道出口流量更接近入口处泵的设定输出流量，设计的装置可用于空间生物技术研究。

Design of space cell culture device and optimization using fluid dynamics simulation

The cell culture apparatus can be used for the research on cell biology, space biotechnology and space medicine. In thisarticle, a flow-loading automatic cell culture device is designed and manufactured, which can be used for automatic vitro cell culture inunderground and space microgravity environment. It can provide periodic and quantitative input of different reagents to the cell cultureplate regularly, and meet the requirements of miniaturization, convenient assembly and high reliability required by space cell culture.The whole device adopts the modular design method, and uses Solid Works software to establish 3D models of basic components, reagentsupply components and culture components, respectively. The Flow Simulation module in Solid Works software is used to conduct finiteelement analysis, fluid simulation, and structural optimization of the pin plate, the key component connected between the cell culturedevice and the cell culture plate. The velocity field and pressure field of the channel inside the pin plate are observed, and the channelis optimized by replacing right angles with rounded corners. The results show that the device is feasible and can meet the requirements ofcell culture device. The fluid stability in the optimized pin-plate channel is stronger, and the flow rate and pressure distribution are moreuniform. In the ground environment, the optimized needle plate has increased the speed uniformity of fluid in the transverse channel by95% , 96% , and 94% , respectively under low, medium, and high flow rates. Similarly, in the longitudinal channel, the speeduniformity has been increased by 68% , 70% , and 72% , respectively under low, medium, and high flow rates. The experimental resultsshow that the optimized flow at the outlet of the pin-plate channel is closer to the set output flow at the inlet pump. The designed devicecan be used in space biotechnology research.