英国红芸豆抗氧化肽对氧化应激斑马鱼的保护作用研究

为探究英国红芸豆抗氧化肽组分(BRKBPAPC)的体内抗氧化作用，利用2,2′-偶氮二异丁基脒二盐酸盐诱导建立氧化应激斑马鱼模型，研究BRKBPAPC对氧化应激斑马鱼的影响。结果表明：BRKBPAPC能显著提高(P<0.05)斑马鱼体内过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力和总抗氧化能力(T-AOC),显著降低(P<0.05)丙二醛(MDA)含量；同时BRKBPAPC还具有调节斑马鱼脂代谢的能力，随着处理浓度的增加斑马鱼脂代谢指标逐渐恢复正常水平；高剂量BRKBPAPC可使斑马鱼行为轨迹增多，高速运动次数、时间、距离显著上升。高剂量抗氧化肽处理21 d时，CAT、SOD、T-AOC、GSH-Px分别增至(28.29±3.29)、(126.59±6.39)、(2.07±0.18)、(84.75±7.77)U/mg prot, MDA含量降至(3.35±0.29)nmol/mg prot。由此可见，BRKBPAPC具有较好的体内抗氧化作用及脂代谢调节能力。

Study on the Protective Effect of British Red Kidney Bean Antioxidant Peptides on Oxidatively Stressed Zebrafish

To investigate the in vivo antioxidant effect of British red kidney bean antioxidant peptide components (BRKBPAPC), an oxidative stress zebrafish model was established using 2,2''-azobis(isobutyl)amidine dihydrochloride induction to investigate the effect of BRKBPAPC on oxidatively stressed zebrafish. The results showed that BRKBPAPC significantly increased (P<0.05) the activity of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC), and significantly decreased (P<0.05) the malondialdehyde (MDA) content in zebrafish, BRKBPAPC also has the ability to regulate zebrafish lipid metabolism, with the increase of treatment concentration zebrafish lipid metabolism index gradually restored to normal level; high dose of BRKBPAPC can make zebrafish behavior trajectory increased, the number of high-speed movement, time, distance significantly increased.At 21 d of high-dose antioxidant peptide treatment, CAT, SOD, T-AOC, and GSH-Px increased to (28.29±3.29),(126.59±6.39),(2.07±0.18),and (84.75±7.77) u/mgprot, MDA content decreased to 3.35±0.29 nmol/mgprot. This shows that BRKBPAPC has good in vivo antioxidant effect and lipid metabolism regulation ability.This study provides a theoretical basis for the development of functional components of British red kidney bean protein.