苦荞籽提取物的抗炎镇痛作用和急性毒性试验研究

目的：研究苦荞籽乙醇提取物的抗炎镇痛作用和急性毒性。方法：采用二甲苯致小鼠耳肿胀法和大鼠蛋清性足肿胀法观察抗炎作用；采用小鼠热板法和醋酸扭体法观察镇痛作用；用最大浓度，最大给药体积的药液灌胃给药。结果：苦荞籽提取物可明显抑制二甲苯所致小鼠耳廓的肿胀度，抑制蛋清所致大鼠足肿胀；可提高热板所致小鼠痛阈值，减少醋酸所致小鼠的扭体次数；最大给药量为374.5g/kg bw，相当于成人日用量的1498 倍。结论：苦荞籽提取物具有明显抗炎镇痛作用，无明显毒性。

Anti-Inflammatory and Analgesic Effect of Ethanolic Extract of Tartary Buckwheat Seed (TSE) and Its Acute Toxicity

The aim of this research was to investigate the anti-inflammatory and analgesic effects and acute toxicity of extract from Tartary Buckwheat seed (TSE). The animal models were established through dimethylbenzene-induced mice and rat inflammatory models with egg white injection under the aponeurosis. The pain models were obtained by hot plate and acetic acid in mice.The maximum administration dosage of TSE were determined via i.g. route in mice. TSE was able to significantly inhibit the mice ear edema induced by dimethylbenzene and the rat hind paw edema induced by egg white. The pain in mice caused by acetic acid and hot plate were evidently suppressed by TSE. The maximum administration dosage was 374.5g of TSE given to mice per kilogram, equivalent to 1498 times of the daily administration dosage of human body. TSE has obvious anti-inflammation and analgesia effect and no apparent toxicity.