牛蒡子中牛蒡子苷的提取分离

以牛蒡子为原料，采用微波辅助提取的方法，对牛蒡子中牛蒡子苷的提取工艺参数进行优化，并采用大孔吸附树脂对牛蒡子苷的粗提液进行初步分离纯化。结果表明，微波辅助提取牛蒡子苷最佳工艺参数为：提取时间80s，微波功率250W，液料比20:1(ml/g)。在此条件下，提取率为9.02%。HPD-700 型大孔吸附树脂对牛蒡子苷的吸附量大、解吸率高，比较适合于牛蒡子苷的分离纯化。HPD-700 型大孔吸附树脂对牛蒡子苷的最佳分离条件为：上样液质量浓度3.26mg/ml，流速1ml/min，洗脱剂为体积分数70% 的乙醇，洗脱流速为1ml/min。

Extraction and Separation of Arctiin from Fructus Arctii

Arctiin, as a precursor of arctigenin which is one of the main functional components in Fructus Arctii, was extracted with ethanol in a microwave field and separated from the extraction liquid with macroporous resin adsorption chromatography. The microwave-assisted extraction procedure of arctiin was optimized using L9(34) orthogonal array design, and the effects of adsorption and desorption conditions on separation of arctiin were discussed. A maximum extraction yield of arctiin of 9.02% was obtained by the 80 s extraction in an 250 W microwave field with a liquid/material ratio of 20:1 (ml/g). Among four types of macroporous resin tested, HPD-700 possessed the best adsorption and desorption performance. The optimum technological parameters for separating arctiin were determined as follows: sample concentration 3.26 mg/ml, flow rate of sample 1 ml/min, and flow rate of 70% ethanol used as an eluant 1 ml/min.