| 原料乳中蜡样芽孢杆菌部分致病基因的鉴定及毒力评价 |
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| 引用本文： | 杨 媛,陈庆森,吴海清. 原料乳中蜡样芽孢杆菌部分致病基因的鉴定及毒力评价[J]. 食品科学, 2009, 30(22): 236-239. DOI: 10.7506/spkx1002-6300-200922053 |
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| 作者姓名： | 杨 媛 陈庆森 吴海清 |
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| 作者单位： | 天津市食品生物技术重点实验室，天津商业大学生物技术与食品科学学院 |
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| 基金项目： | “十一五”国家科技支撑计划项目(2006BAD04A08) |
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| 摘 要： | 研究原料乳中蜡样芽孢杆菌的潜在威胁，对我国10 个省市原料乳中蜡样芽孢杆菌的污染状况进行调查，并利用PCR 扩增技术及血平板检测的方法分析乳源性蜡样芽孢杆菌中的毒性分布。结果表明，原料乳中蜡样芽孢杆菌检出率为33%，检出水平为1 × 103～1 × 106 CFU/ml，检出的蜡样芽孢杆菌中25 株(75.8%)能产生溶血素BL，24 株(72.7%)含有bceT 基因，至少产生一种毒素的菌株有31 株占检出菌总数的93.9%。研究结果证实我国原料乳中的蜡样芽孢杆菌污染状况不容乐观，严格监控原料乳中的蜡样芽孢杆菌污染，对生产出优质乳制品具有重要意义。
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| 关 键 词： | 原料乳 蜡样芽孢杆菌 溶血素BL hblA 基因 bceT 基因 |
| 收稿时间： | 2009-07-06 |
Toxigenic Potential of Bacillus cereus in Row Milk |
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| YANG Yuan,CHEN Qing-sen,WU Hai-qing. Toxigenic Potential of Bacillus cereus in Row Milk[J]. Food Science, 2009, 30(22): 236-239. DOI: 10.7506/spkx1002-6300-200922053 |
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| Authors: | YANG Yuan CHEN Qing-sen WU Hai-qing |
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| Affiliation: | Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science,Tianjin University of Commerce, Tianjin 300134, China |
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| Abstract: | In order to evaluate the toxigenic potential of Bacillus cereus in row milk, 100 samples of row milk from 10 cities of China were screened, and Bacillus cereus were found in 33 (33%) samples with the concentration within 1×103～1×106 CFU/ ml. Using PCR the isolates were checked for the presence of the hblA gene of the hemolysin BL (HBL) complex and the bceT genes of the enterotoxin T. HBL was detected by blood plate. Totally 25 (75.8%) of the 33 B. cereus isolates produced the hemolysin BL, while 24 (72.7%) isolates possessed the bceT gene, and as a result, 31(93.9%) B. cereus isolates produced at least one toxin. The results suggest that it is necessary to monitor the Bacillus cereus contamination in row milk. |
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| Keywords: | raw milk Bacillus cereus hemolysin BL hblA gene bceT gene |
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