羧甲基壳聚糖与阿霉素联合对Hela 细胞的抑制作用研究

目的：探讨羧甲基壳聚糖与阿霉素联合应用对人宫颈癌Hela 细胞增殖的影响。方法：采用四甲基偶氮唑蓝(MTT)比色法测定单独和合用羧甲基壳聚糖与阿霉素对癌细胞的生长抑制作用，应用金氏公式进行联合用药分析。结果：羧甲基壳聚糖和阿霉素均可有效的抑制癌细胞生长，且同时给药对癌细胞可产生单纯相加至增强的协同杀伤效果。序贯给药法中先给阿霉素后给羧甲基壳聚糖结果为协同作用，先给羧甲基壳聚糖后给阿霉素为拮抗作用。结论：羧甲基壳聚糖与阿霉素同时联合用药可产生协同作用，序贯联合用药产生单向协同作用。

Inhibitory Effect of Combined Administration of Carboxymethyl-chitosan and Adriamycin on Hela Cells

Objective: To investigate the effect of combined administration of carboxymethyl chitosan (CM-CTS) and adriamycin (ADR) on proliferation of human cervical carcinoma cell line (Hela) in vitro. Methods: Effects of both drugs on proliferation of tumor cells were evaluated by MTT assay. Results: Alone administration of CM-CTS or ADR revealed an inhibitory effect on proliferation of tumor cells and a synergistic effect on inhibition of tumor cell proliferation was observed under simultaneous administration of both drugs. In addition, sequential administration of ADR followed by CM-CTS had a synergistic effect; in contrast, sequential administration of CM-CTS followed by ADR exhibited an antagonistic effect. Conclusion: Simultaneous administration of CM-CTS and ADR revealed a synergistic effect and sequential administration of CM-CTS and ADR exhibited an unidirectional synergistic effect.