环丙沙星免疫亲和柱的制备及优化

制备环丙沙星(Cip)抗生素的免疫亲和柱(IAC)，研究优化IAC 柱的鉴定和洗脱条件，并测试其最佳性能，最终洗脱液选择4ml 50% 的甲醇，在重复使用5 次后，其柱容量仍能达到开始柱容量的25% 左右，采用高效液相色谱荧光检测器(HPLC-FLD)对IAC 进行回收率测定，以0.05% 的甲酸水溶液- 甲醇体系作为流动相，梯度洗脱HPLC-FLD 方法的线性范围10～1000ng/ml，相关系数大于0.99，测定出IAC 柱对环丙沙星的回收率为74.38%～105.552%。

Preparation and Optimization of Immunoaffinity Column for Ciprofloxacin

In order to prepare optimal immunoaffinity column (IAC) for ciprofloxacin, elution parameters and separation efficiency were systematically analyzed. The excellent separation performance was observed using 4 ml of 50% methanol in PBS as elutent. Column capacity remained 25% of original capacity after 5 cycles of use at the optimized condition. Recovery rate determination was conducted using high performance liquid chromatography with a fluorescence detector (HPLC-FLD) under the gradient elution through 0.05% formic acid aqueous solution-methanol. The HPLC-FLD method exhibited an excellent linear in the range of 10－1000 ng/ml with a correlation coefficient of 0.99. Recovery rate of ciprofloxacin using IAC was 74.38%－ 105.552%.