| 应用大孔树脂纯化花生壳总黄酮 |
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| 引用本文： | 杨庆利,毕 洁,禹山林,张吉民,宫清轩,秦蒙蒙. 应用大孔树脂纯化花生壳总黄酮[J]. 食品科学, 2009, 30(20): 44-48. DOI: 10.7506/spkx1002-6300-200920004 |
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| 作者姓名： | 杨庆利 毕 洁 禹山林 张吉民 宫清轩 秦蒙蒙 |
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| 作者单位： | 1.山东省花生研究所 2.青岛大学医学院 |
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| 基金项目： | 国家高技术研究发展计划项目(2007AA10Z189 2006AA10A114);"十一五"国家科技支撑计划项目(2008BAD97B04);现代农业产业技术体系建设专项(nycytx-19);农业部公益性行业（农业）科研专项经费资助项目(nyhyzx07-0140) |
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| 摘 要： | 研究大孔树脂纯化花生壳总黄酮的工艺条件,对大孔树脂的种类及其静态吸附、解吸附条件进行初步探讨。通过静态吸附和解吸附的比较,从7种不同型号的大孔吸附树脂中选出AB-8、DM301、NKA-9三种树脂进行静态吸附解吸动力学,发现NKA-9的吸附解吸效果较为稳定,其吸附解吸平衡时间分别为5h和2h。通过单因素试验,NKA-9的最佳吸附条件为35℃、样液pH7.5,样液中花生壳总黄酮初始浓度(0.112±0.02)mg/ml;最佳解吸条件为体积分数90%乙醇作为解吸液、解吸液用量15ml/g湿树脂、解吸液pH8.5。
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| 关 键 词： | 大孔树脂 花生壳 总黄酮 纯化 |
| 收稿时间： | 2009-07-19 |
Use of Macroporous Resin for Purification of Total Flavonoids from Peanut Hull |
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| YANG Qing-li BI Jie,YU Shan-lin ZHANG Ji-min GONG Qing-xuan QIN Meng-meng. Use of Macroporous Resin for Purification of Total Flavonoids from Peanut Hull[J]. Food Science, 2009, 30(20): 44-48. DOI: 10.7506/spkx1002-6300-200920004 |
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| Authors: | YANG Qing-li BI Jie YU Shan-lin ZHANG Ji-min GONG Qing-xuan QIN Meng-meng |
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| Affiliation: | 1. Shandong Peanut Research Institute, Qingdao 266100, China2. Qingdao University Medical College, Qingdao 266071, China |
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| Abstract: | AB-8, DM301 and NKA-9 resins were screened from 7 types of macroporous resin in terms of adsorption and desorption capacities to total flavonoids from peanut hull for further studies of static adsorption and desorption kinetics. NKA-9 resin was finally chosen for the purification due to better adsorption and desorption performance, whose equilibrium time of adsorption and desorption were 5 and 2 h, respectively. Single factor experiments showed that the optimum purification procedure of total flavonoids from peanut hull was based on adsorption at 35 ℃of sample solution with an initial concentration of 0.112 ± 0.02 mg/ml at pH 7.5, followed by desorption using 90% aqueous ethanol (V/V) at pH 8.5 with an amount of 15 ml/g (wet resin). |
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| Keywords: | macroporous resin peanut hull total flavonoids purification |
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