| SD3 离子交换树脂分离提取ATP 的研究 |
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| 引用本文： | 乔德阳,甘 聃. SD3 离子交换树脂分离提取ATP 的研究[J]. 食品科学, 2009, 30(6): 128-131. DOI: 10.7506/spkx1002-6630-200906026 |
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| 作者姓名： | 乔德阳 甘 聃 |
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| 作者单位： | 徐州工业职业技术学院化学工程系 |
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| 摘 要： | 通过对SD3 离子交换树脂对ATP 动态吸附、洗杂和洗脱条件的考察，确定最适吸附条件为：样品溶液H 2.0，浓度8.25g/L，离子交换柱的高径比为10(装柱湿树脂80g)，流速1.0ml/min；最优洗杂液为pH1.5 HC1＋ 0.05mol/L NaCl 的溶液；ATP 的最优洗脱液为pH12.0 NaOH+0.1mol/L NaCl 溶液，优洗脱速度为0.5ml/min。
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| 关 键 词： | 三磷酸腺苷(ATP) 离子交换树脂 分离 |
| 收稿时间： | 2008-12-30 |
Separation of ATP from Waste Beer Yeast Hydrolysate by SD3 Ion Exchange Resin |
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| QIAO De-yang,GAN Dan. Separation of ATP from Waste Beer Yeast Hydrolysate by SD3 Ion Exchange Resin[J]. Food Science, 2009, 30(6): 128-131. DOI: 10.7506/spkx1002-6630-200906026 |
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| Authors: | QIAO De-yang GAN Dan |
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| Affiliation: | (Department of Chemical Engineering, Xuzhou College of Industrial Technology, Xuzhou 221140, China) |
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| Abstract: | The dynamic adsorption and desorption of SD3 ion exchange resin to ATP were studied. The results showed that the optimum adsorption conditions of ATP are as follows: pH of waste beer yeast hydrolysate 2.0, its concentration 1.0 mg/ml, its flow rate 8.25 g/L and diameter to height ratio of column 10 (80 g of wet resin filled into the column). The optimum eluent for removing impurity is HCl solution at pH 1.5 containing 0.05 mol/L NaCl. And the optimum elution conditions of ATP are as follows: taking NaOH solution at pH 12.0 containing 0.1 mol/L NaCl as eluent and its flow rate 0.5 ml/min. |
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| Keywords: | ATP ion exchange resin separation |
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