| 饮料中天冬酰苯丙氨酸甲酯及其代谢物含量的测定方法 |
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| 引用本文： | 李 皓,宋阳成,滕红刚,陈宇飞,周 全,陈长武.饮料中天冬酰苯丙氨酸甲酯及其代谢物含量的测定方法[J].食品科学,2009,30(18):249-251. |
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| 作者姓名： | 李 皓 宋阳成 滕红刚 陈宇飞 周 全 陈长武 |
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| 作者单位： | 1.吉林工程技术师范学院 2.吉林亚泰生物药业股份有限公司 3. 吉林工商学院 |
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| 摘 要： | 目的：建立快速测定饮料中天冬酰苯丙氨酸甲酯及其代谢物(L- 苯丙氨酸)含量的系统分析方法。方法：采用高效液相色谱法，使用ZORBAX SB-C18 色谱柱，以0.1% 三氟乙酸- 乙腈流动相(85:15，V/V)作为分离条件，在DAD 检测器210nm 波长处进行检测。结果：该方法的最低检出量分别为L- 苯丙氨酸0.8ng，天冬酰苯丙氨酸甲酯2.4ng，标准曲线线性良好，相关系数分别为0.9998 和0.9994，回收率在96.3%～98.5% 之间，相对标准偏差小于2%。结论：该法简捷、快速且灵敏度高。
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| 关 键 词： | 饮料 天冬酰苯丙氨酸甲酯 苯丙氨酸 高效液相色谱法 |
| 收稿时间： | 2009-06-10 |
HPLC Determination of Aspartame and Its Metabolite in Commercial Beverages |
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| LI Hao,SONG Yang-cheng,TENG Hong-gang,CHEN Yu-fei,ZHOU Quan,CHEN Chang-wu.HPLC Determination of Aspartame and Its Metabolite in Commercial Beverages[J].Food Science,2009,30(18):249-251. |
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| Authors: | LI Hao SONG Yang-cheng TENG Hong-gang CHEN Yu-fei ZHOU Quan CHEN Chang-wu |
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| Affiliation: | 1. Jilin Teacher＇s Institute of Engineering and Technology, Changchun 130052, China；2. Jilin Yatai Biopharmaceuticals Co. Ltd.,
Changchun 130032, China；3. Jilin Business and Technology College, Changchun 130062, China |
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| Abstract: | A HPLC method was presented for the rapid determination of aspartame and its metabolite in commercial beverages. The analytes were separated on a ZORBAX SB-C18 column using a mobile phase composed of 0.1% trifluoroacetic acid and acetonitrile (85:15, V/V) and detected using a DAD detector at 210 wavelength. The limits of detection of phenylalanine and aspartame by the method were 0.8 and 2.4 ng, respectively. There was a good linear relationship between concentration and peak area, and the correlation coefficients were 0.9998 and 0.9994, respectively. The spiked recoveries were 96.3% and 98.5%, respectively, with a RSD less than 2%. The method is simple, rapid and highly sensitive. |
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| Keywords: | beverage aspartame phenylalanine HPLC |
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