| 转谷氨酰胺酶在尼龙网上的固定化及其酶学性质的研究 |
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| 引用本文： | 刘 颖,张 鹤,石彦国,马永强,张 丹. 转谷氨酰胺酶在尼龙网上的固定化及其酶学性质的研究[J]. 食品科学, 2009, 30(23): 290-293. DOI: 10.7506/spkx1002-6300-200923065 |
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| 作者姓名： | 刘 颖 张 鹤 石彦国 马永强 张 丹 |
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| 作者单位： | 哈尔滨商业大学食品工程学院 |
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| 基金项目： | 国家“863”计划项目(2006AA10Z329) |
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| 摘 要： | 以硫酸二甲酯活化后的尼龙网为载体，戊二醛为交联剂固定转谷氨酰胺酶，并对固定化条件及固定化酶的酶学性质进行研究。结果表明固定化的最适条件为：1cm2 处理后的尼龙网与20ml 7% 的戊二醛交联5h 后，在4℃条件下固定10ml 1.5mg/ml 的转谷氨酰胺酶溶液6h，此条件下酶活回收率达到46%。固定化转谷氨酰胺酶相对于游离酶具有更好的热稳定性，并具有良好的储存性及操作稳定性。
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| 关 键 词： | 转谷氨酰胺酶 固定化 尼龙网 |
| 收稿时间： | 2009-08-11 |
Immobilization and Enzymatic Properties of Transgluminase on Nylon Net |
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| LIU Ying,ZHANG He,SHI Yan-guo,MA Yong-qiang,ZHANG Dan. Immobilization and Enzymatic Properties of Transgluminase on Nylon Net[J]. Food Science, 2009, 30(23): 290-293. DOI: 10.7506/spkx1002-6300-200923065 |
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| Authors: | LIU Ying ZHANG He SHI Yan-guo MA Yong-qiang ZHANG Dan |
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| Affiliation: | College of Food Engineering, Harbin Commercial University, Harbin 150076, China |
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| Abstract: | Transgluminase was immobilized on nylon net using activated dimethylsulfate as the carrier and glutaraldehyde as the cross-linking agent. Immobilization conditions and enzymatic properties of immobilized enzyme were also investigated. The optimal condition for immobilization was initial crosslinkage reaction of nylon net with 7% glutaraldehyde for 5 hours and final immobilization reaction using 10 ml of 1.5 mg/ml transgluminase on activated carrier for 6 hours. Activity of immobilized transgluminase was 46% under this optimal immobilization condition. Compared to free transgluminase, immobilized transgluminase exhibited better thermo-stability during storage and experimental operations. |
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| Keywords: | transglutaminase immobilization nylon net |
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