| 孔雀石绿单克隆抗体的制备及鉴定 |
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| 引用本文： | 李招慧,姜盼盼,何计国.孔雀石绿单克隆抗体的制备及鉴定[J].食品科学,2009,30(13):223-226. |
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| 作者姓名： | 李招慧 姜盼盼 何计国 |
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| 作者单位： | 中国农业大学食品科学与营养工程学院 |
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| 摘 要： | 以合成的孔雀石绿- 牛血清白蛋白(MG-BSA)为免疫原免疫Balb/c 小鼠，利用杂交瘤细胞技术，建立1 株稳定分泌孔雀石绿单克隆抗体的杂交瘤细胞5G6，其分泌的抗体亚类为IgG1，轻链为κ型。间接ELISA 测定腹水效价达1.28 × 105。间接竞争ELISA(ciELISA)显示其对孔雀石绿的50% 抑制浓度(IC50)为1.36ng/ml，除结晶紫(CV)外，与无色孔雀石绿(LMG)没有交叉反应。
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| 关 键 词： | 孔雀石绿 单克隆抗体 酶联免疫 |
| 收稿时间： | 2009-04-08 |
Preparation and Identification of Monoclonal Antibody against Malachite Green |
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| LI Zhao-hui,JIANG Pan-pan,HE Ji-guo.Preparation and Identification of Monoclonal Antibody against Malachite Green[J].Food Science,2009,30(13):223-226. |
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| Authors: | LI Zhao-hui JIANG Pan-pan HE Ji-guo |
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| Affiliation: | College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083,China |
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| Abstract: | Balb/c Mice were immunized with the malachite green (MG) coupled with bovine serum albumin, and a strain of hybridoma cell named 5G6, which can stably secret monoclonal antibody (McAb), were obtained by cell hybridoma technology. The subclass of the McAb was IgG1, and the light chain was κ type. The ascites titer determined by indirect ELISA was 1.28 ×105, while the indirectly competitive ELISA showed that its IC50 to malachite green was 1.36 ng/ml. Except crystal violet (CV), the McAb had no cross-reaction with leucomalachite green (LMG). |
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| Keywords: | malachite green (MG) monoclonal antibody (McAb) enzyme-linked immune sorbent assay |
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