金银花中绿原酸和黄酮的同时提取分离工艺研究

采用乙醇超声波法提取，聚酰胺柱层析法分离的方法对金银花中的绿原酸和黄酮进行提取分离。结果表明：聚酰胺柱层析法分离、梯度洗脱得到绿原酸、水溶性黄酮和脂溶性黄酮3个不同产品A、B和C，其中A绿原酸含量为68.2%，得率为1.34%；B黄酮含量95%，得率为1.12%；C中槲皮素和木犀草素含量分别为7.5%、16.9%，得率为0.11%。

Simultaneous Extraction and Isolation of Chlorogenic Acid and Flavonoids from Flos lonicerae

Chlorogenic acid and flavonoids were simultaneously extracted by ethanol with the aid of ultrasonic treatment from Flos lonicerae and then isolated by polyamide column chromatography. Results showed that three products (A, B and C) were obtained from column chromatography with gradient elution, which were identified to be chlorogenic acid, soluble flavonoid and lipid-soluble flavonoid, respectively. The chlorogenic acid content in product A was 68.2% with a yield of 1.34%; the flavonoid content in product B was 95.0% with a yield of 1.12%; and the quercetin and lonicertin contents in product C were 7.5 and 16.9%, respectively, with a yield of 0.11%.