高效液相色谱法测定银杏保健品中总黄酮

目的：建立一种适用性好，能作为国家标准方法用于测定银杏保健品中总黄酮化合物的测定法。方法：对盐酸浓度、水解时间、水解温度三因素采用响应曲面优化保健品中黄酮化合物水解提取条件；采用高效液相色谱方法，梯度洗脱程序测定黄酮类化合物含量。结果：水解最佳条件为水解时间114min，水解温度79℃，盐酸浓度3.4mol/L，该条件下得到的银杏总黄酮含量最高为8851.468mg/100g；流动相 A：0.1% H3PO4，B：CH3OH；梯度洗脱程序为：0～20min，A 45%，B 55%；20～30min，A 由45% 线性变化为50%。该法线性关系良好，平均加标回收率在95.04%～104.56% 之间，RSD 小于2%。结论：该法操作简单、快速、分离效果好、专属性强、准确度和重现性好、适用性好，可作为国家标准方法推荐使用。

Determination of Total Flavonoids in Ginkgo biloba Health Products by HPLC

A more applicable method was developed to determine the total flavonoids in Ginkgo biloba health products by HPLC in this study. The effects of the concentration of HCl, temperature and hydrolysis time on the extraction yield of total flavonoids from Ginkgo biloba health products were studied by response surface methodology (RSM). The samples were determined by HPLC with gradient elution. The optimized condition for hydrolysis was: 114 min, 79 ℃ and 3.4 mol/L of HCl. This optimum condition led to the flavonoid yield of 8851.468mg/100g. This method showed good linearity and recovery recoveries (95.04%－104.56%) as well as low RSDs (＜2.0%). This simple, rapid, reproducible method is regarded to be very useful for evaluating the quality of the total flavanoids in Ginkgo biloba health products.