| 庆大霉素酶联免疫检测法研究 |
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| 引用本文： | 马 伟,汪宝欢,李 娟,胥传来. 庆大霉素酶联免疫检测法研究[J]. 食品科学, 2009, 30(10): 242-244. DOI: 10.7506/spkx1002-6630-200910057 |
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| 作者姓名： | 马 伟 汪宝欢 李 娟 胥传来 |
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| 作者单位： | 江南大学食品学院 |
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| 摘 要： | 本实验采用戊二醛方法合成庆大霉素的免疫原，利用凝胶电泳对免疫原进行鉴定。使用该免疫原免疫兔子，得到抗血清测出效价高于512000，对抗血清进行纯化测出检测限达0.1ng/ml，建立庆大霉素的免疫检测方法。
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| 关 键 词： | 庆大霉素 抗体 免疫检测 |
| 收稿时间： | 2008-10-30 |
Development of Enzyme-linked Immunoassay for Detection of Gentamicin |
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| MA Wei,WANG Bao-huan,LI Juan,XU Chuan-lai. Development of Enzyme-linked Immunoassay for Detection of Gentamicin[J]. Food Science, 2009, 30(10): 242-244. DOI: 10.7506/spkx1002-6630-200910057 |
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| Authors: | MA Wei WANG Bao-huan LI Juan XU Chuan-lai |
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| Affiliation: | (School of Food Science and Technology, Jiangnan University, Wuxi 214122, China) |
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| Abstract: | An immunogen, gentamicin-bovine serum albumin (BSA), was prepared by glutaraldehyde coupling method, and was identified by SDS-PAGE. New Zealand white rabbit was immunized with this immunogen three times. After immunization the antiserum with the titer of higher than 512000 was obtained. After purified by salting-out with ammonium sulfate, the obtained anti- gentamicin antibody was used to develop an indirect enzyme-linked immunosorbent assay (ELISA) for detection of gentamicin, with the detection limit of 0.1 ng/ml. |
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| Keywords: | gentamicin antibody immunoassy |
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