乙醇浸提法制备菜籽浓缩蛋白的工艺研究

本实验采用乙醇浸提法制备菜籽浓缩蛋白，在单因素试验的基础上，采用L9(34)正交优化试验，探讨乙醇浓度、浸提温度、浸提次数和料液比对浓缩蛋白产品蛋白含量的影响。单因素试验结果表明，最佳浸提工艺参数分别为：乙醇浓度70%、浸提温度60℃、浸提次数5 次，料液比1:7、每次浸提25min。正交试验结果表明：影响蛋白含量的主要因素为乙醇浓度，其次为浸提温度、料液比、浸提次数。最佳浸提工艺条件为A3B2C2D2，即乙醇浓度70%、浸提温度55℃、料液比1:6，浸提次数5 次。在此条件下制备的浓缩蛋白产品蛋白含量为60.5%，浓缩蛋白得率为75.7%，浓缩蛋白产品中抗营养因子成分——单宁、植酸、硫甙分别被脱除90.5%、66.7%、99.3%。

Study on Alcohol Extraction Process of Protein Concentrate from Rapeseed Meal

In alcohol extraction process of rapeseed protein concentrate (RPC), the effects of alcohol concentration, extraction temperature, extraction times and ratio of solid to liquid on the protein content in extract were investigated, and these parameters of extraction process were optimized through orthogonal test. The orthogonal test results indicated that, the most significant factor which affects the protein content is alcohol concentration, followed by extraction temperature, solid-liquid rate, and extraction times in turn. The best extraction condition combination is A3B2C2D2, namely alcohol concentration 70%, extraction temperature 55 ℃, ratio of solid to liquid 1:6, extraction times 5. Under these conditions, the content and the yield of protein are 60.5% and 75.7% respectively, and the removal rates of the anti-nutritional compounds such as tannin, phytic acid and glucosinolate are 90. 5% , 66.7% and 99.3% respectively.