| 米诺环素完全抗原的制备与表征 |
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| 引用本文： | 纪淑娟,郭德超,杜阿楠.米诺环素完全抗原的制备与表征[J].食品科学,2009,30(21):305-308. |
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| 作者姓名： | 纪淑娟 郭德超 杜阿楠 |
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| 作者单位： | 沈阳农业大学食品学院 |
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| 基金项目： | 辽宁省教育厅重点项目(2009A633) |
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| 摘 要： | 研究制备能有效用于米诺环素(MNC)残留检测的完全抗原。采用戊二醛合成法和重氮化合成法，将米诺环素与牛血清白蛋白(BSA)偶联，分别制备MNC 完全抗原MNC-BSA。经红外和紫外光谱扫描结果表明：两种物质已经偶联，两种完全抗原的平均偶联比分别为9.9 和5.9。采用戊二醛法合成的完全抗原免疫兔，检测抗血清效价为1:16000，表明合成的完全抗原可以作为免疫原用于制备抗体及检测时的包被原进行ELISA 检测。
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| 关 键 词： | 米诺环素 牛血清白蛋白 偶联 完全抗原 |
| 收稿时间： | 2009-08-01 |
Preparation and Characterization of Complete Antigen of Minocycline |
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| JI Shu-juan,GUO De-chao,DU A-nan.Preparation and Characterization of Complete Antigen of Minocycline[J].Food Science,2009,30(21):305-308. |
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| Authors: | JI Shu-juan GUO De-chao DU A-nan |
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| Affiliation: | College of Food Science, Shenyang Agricultural University, Shenyang 110161, China |
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| Abstract: | In order to effectively detect minocycline (MNC) residues, a complete antigen of MNC was developed by glutaraldehyde synthesis and diazotization methods. MNC was coupled with bovine serum albumin (BSA) to obtain a complete antigen of MNC-BSA. Infrared and ultraviolet scanning spectral analysis confirmed that the complete antigen of MNC-BSA had been successfully coupled by both methods, with average conjugation ratios of 9.9 and 5.9, respectively. The serum titer of rabbit immunized with MNC-BSA competent antigen prepared by glutaraldehyde synthesis method was 16000, which indicated that the synthesized complete antigen could not only be used as the immunogen to prepare the corresponding antibody, but also act as the coating antigen for enzyme linked immunosorbent assay (ELISA) kit. |
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| Keywords: | minocycline bovine serum albumin (BSA) conjugation complete antigen |
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