| 液体深层发酵条件对银耳菌丝体酸性多糖富集的影响 |
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| 引用本文： | 王瑞雪,颜 军,邬晓勇,何 钢,张良蕾,姚尊伟,苟小军.液体深层发酵条件对银耳菌丝体酸性多糖富集的影响[J].食品科学,2009,30(21):175-177. |
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| 作者姓名： | 王瑞雪 颜 军 邬晓勇 何 钢 张良蕾 姚尊伟 苟小军 |
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| 作者单位： | 1.四川抗菌素工业研究所 2.成都大学中药化学实验室 |
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| 基金项目： | 四川省中医药管理局科技专项资助项目(2008-01)；四川省教育厅科研项目(08ZA173) |
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| 摘 要： | 考察液体发酵培养基中不同碳源、碳源用量以及培养基pH 值对银耳菌丝体富集酸性多糖的影响。结果表明：1% 的蔗糖、pH 值为7.0 的培养基有利于银耳菌丝体酸性多糖的富集。并对所得银耳多糖进行DEAE-sepherosefast flow 柱层析，发现菌丝体与子实体相比，多糖组成发生显著改变。
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| 关 键 词： | 银耳 菌丝体 酸性多糖 液体深层发酵 |
| 收稿时间： | 2009-06-16 |
Effects of Submerged Fermentation on Acidic Polysaccharides in Tremella fuciformis Mycelia |
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| WANG Rui-xue,YAN Jun,WU Xiao-yong,HE Gang,ZHANG Liang-lei,YAO Zun-wei,GOU Xiao-jun.Effects of Submerged Fermentation on Acidic Polysaccharides in Tremella fuciformis Mycelia[J].Food Science,2009,30(21):175-177. |
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| Authors: | WANG Rui-xue YAN Jun WU Xiao-yong HE Gang ZHANG Liang-lei YAO Zun-wei GOU Xiao-jun |
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| Affiliation: | 1. Sichuan Industrial Institute of Antibiotics, Chengdu 610052, China；2. Laboratory for Chemistry of Traditional Chinese
Medicine, Chengdu University, Chengdu 610106, China |
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| Abstract: | This paper mainly deals with effects of different carbon sources, carbon source amount and medium pH on accumulation of acidic polysaccharides in Tremella fuciformis mycelia during submerged fermentation. Along with this, the fractionation of the polysaccharides by DEAE Sepharose Fast Flow chromatography is also discussed in this paper. Results showed that the fermentation medium containing 1% sucrose at pH 7.0 was conducive to the enrichment of acidic polysaccharides. The distribution of polysaccharide in mycelia and fruit body of Tremella fuciformis revealed a significant change. |
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| Keywords: | Tremella fuciformis mycelium acidic polysaccharide submerged fermentation |
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