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Saliva improves Streptococcus mitis protective effect on human gingival fibroblasts in presence of 2-hydroxyethyl-methacrylate
Authors:Mara Di Giulio  Viviana di Giacomo  Emanuela Di Campli  Soraya Di Bartolomeo  Susi Zara  Guido Pasquantonio  Amelia Cataldi  Luigina Cellini
Affiliation:1. Department of Pharmacy, University “G. d’Annunzio”, Chieti-Pescara, Via dei Vestini, 31, Chieti, Italy
2. Department of Odontostomatological Sciences, University of Roma “Tor Vergata”, Rome, Italy
Abstract:This study aimed to investigate the effect of saliva on Streptococcus mitis free cells and on S. mitis/human gingival fibroblasts (HGFs) co-culture model, in presence of 2-hydroxyethyl-methacrylate (HEMA). The bacterial aggregation both in the planktonic phase and on HGFs, as well as the apoptotic and necrotic eukaryotic cells amount were analyzed, in presence of saliva and/or HEMA. The aggregation test revealed a significant saliva aggregation effect on S. mitis strains compared to the untreated sample. No significant differences were recorded in the amount of culturable bacteria in all studied conditions; however, from microscopy images, the saliva/HEMA combining effect induced a significant bacterial aggregation and adhesion on HGFs. HEMA treatment decreased viable eukaryotic cell number with a parallel increment of necrotic cells, but when saliva was added to the co-culture, the viable cells percentage increased to a value comparable to the control sample.
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