| 三裂叶葛愈伤组织形成和异黄酮类的产生 |
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| 引用本文： | 李玲,刘慧丽,史永忠. 三裂叶葛愈伤组织形成和异黄酮类的产生[J]. 高技术通讯, 2001, 11(5): 25-27 |
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| 作者姓名： | 李玲 刘慧丽 史永忠 |
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| 作者单位： | 华南师范大学生物系广州 510631 |
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| 基金项目： | 教育部优秀青年教师资助项目，广东省自然科学基金资助项目 ( 9942 16) |
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| 摘 要： | 三裂叶葛无菌苗器官在MS 1mg/LNAA 1mg/L2,4,5-T培养基上培养，皆能形成愈伤组织，以子叶的诱导频率最高，B5 1mg/12,4D 0.1mg/LNAA 300mg/L水解酪蛋白用于愈伤组织继代培养，愈伤组织颗粒不断扩大，保持淡绿色。不同外植体形成的愈伤组织经继代培养产生异黄酮和葛根素的能力不同。
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| 关 键 词： | 三裂叶葛 愈伤组织 异黄酮类 药用植物 葛根素 |
Calli Formation and Isoflavonoid Production ofPueraria phaseoloides (Roxb.) Benth. |
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| Li Ling,Liu Huili,Shi Yongzong. Calli Formation and Isoflavonoid Production ofPueraria phaseoloides (Roxb.) Benth.[J]. High Technology Letters, 2001, 11(5): 25-27 |
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| Authors: | Li Ling Liu Huili Shi Yongzong |
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| Abstract: | It was found that organs of sterile seedlings of Pueraria phaseoloides (Roxb.) Benth. formed Calli on MS medium contained 1 mg/L NAA and 1mg/L 2,4,5 T for 20 days, the callus induced frequency of cotyledon was higher than those frequency of leaves, stems, roots, embryonal axis and radicle . The callus growth increased on a subculture medium consisting B5 salts supplemented with 1mg/L 2,4 D, 0.1 mg/L NAA ,300mg/L casein hydrolysate and adjusted to pH 6.0. The production capacity of isoflavone and puerarin in calli formed from several organs were different. |
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| Keywords: | Pueraria phaseoloides (Roxb.) Benth Callus |
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