紫花苜蓿酶解光合生物制氢工艺正交优化实验

为了研究紫花苜蓿酶解液作为光合制氢原料时的最佳产氢工艺条件,对产氢影响较为显著的温度、光照强度、初始pH值三种因素设计了三因素三水平L9(33)正交实验,并对实验结果进行直观分析与方差分析,以获取最佳产氢工艺条件。实验结果表明:在所选水平范围内,各因素对能源草产氢的影响主次顺序为温度→光照强度→初始pH值;由方差分析可知,温度和光照强度对能源草光合产氢性能影响为显著;初始pH值为不显著;由各因素水平值的均值可见,能源草光合生物制氢的最佳工艺水平为30℃、pH=7、光照度为3 000 lx。

Orthogonal optimization experiment of photo-hydrogen production of alfalfa pretreated by enzymatic hydrolysis

In order to study the best technological conditions of enzymolysis liquid of alfalfa as raw materials for photo-hydrogen production, A three factor and three level L9(33) orthogonal experiment is used the three factors are :light, temperature, and initial pH as they have the most significant impact on hydrogen production. And conduct sintuitive and variance analysis with the results of the orthogonal experiment to get the best hydrogen production process conditions. The results of the orthogonal experiment showed that, in the level range as taken, the primary and secondary sequence of impact over hydrogen production for energy grass was temperature, light and initial pH. And the analysis of variance suggested that the impact of temperature and light were significant,while the impact of initial pH was non-significant. According to the average level of various factors, the optimum technical conditions was temperature 30°C, pH 7, illuminance 3000lx.