Rapid ethanolysis procedure for jojoba wax analysis by gas liquid chromatography |
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| Authors: | C. C. Duncan D. M. Yermanos J. Kumamoto C. S. Levesque |
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| Affiliation: | (1) Department of Plant Sciences, University of California, 92502 Riverside, California |
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| Abstract: | A rapid ethanolysis procedure for preparing jojoba wax for gas liquid chromatographic analysis is described. The wax esters are hydrolyzed by refluxing 4 drops of jojoba wax in 5% HCl in anhydrous ethanol in a test tube. The resulting fatty acid ethyl esters and fatty alcohols are separated and quantitated by a single gas liquid chromatographic run. Analysis of duplicate samples by this procedure gives essentially the same results as a procedure which requires 10 times more sample and reagents and considerably more time. |
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