| 废次烟叶中绿原酸的提取与分离 |
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| 引用本文： | 古君平,魏万之. 废次烟叶中绿原酸的提取与分离[J]. 烟草科技, 2010, 0(2) |
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| 作者姓名： | 古君平 魏万之 |
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| 作者单位： | 湖南大学化学生物传感与计量学国家重点实验室,长沙市岳麓区麓山南路2号,410082 |
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| 摘 要： | 考察了提取溶剂种类、乙醇浓度、料液比、提取时间、提取溶剂pH值,以及HPD-400大孔树脂吸附与脱附条件对废次烟叶中绿原酸提取与分离的影响。结果表明:①超声辅助提取的适宜条件为:提取溶剂30%(pH4)乙醇,料液比1∶10,提取时间45min;②烟叶绿原酸提取液的层析条件为:层析柱:HPD-400大孔树脂柱;上柱液浓度4.0mg/mL;上柱液体积:42.5mL;流速1.42mL/min;洗脱液191.7mL30%乙醇水溶液。采用此条件,10.1g废次烟叶样品可提取0.335g固形物,其绿原酸含量为31.9%,绿原酸提取率为1.71%,层析回收率72.3%。
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| 关 键 词： | 废次烟叶 绿原酸 HPD-400大孔树脂 |
Extraction and Separation of Chlorogenic Acid from Waste and Sub-standard Tobacco |
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| GU JUN-PING,WEI WAN-ZHI. Extraction and Separation of Chlorogenic Acid from Waste and Sub-standard Tobacco[J]. Tobacco Science & Technology, 2010, 0(2) |
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| Authors: | GU JUN-PING WEI WAN-ZHI |
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| Affiliation: | GU JUN-PING , WEI WAN-ZHI State Key Laboratory of Chemo/Biosensing , Chemometrics,Hunan University,Changsha 410082,China |
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| Abstract: | |
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| Keywords: | Waste and sub-standard tobacco Chlorogenic acid HPD-400 macroporous adsorptive resin |
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