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树脂对小麦秸秆水解液中丁醇发酵抑制物的脱除效果
引用本文:刘国强,易卓林,何开泽,杨林,方扬,赵海,靳艳玲. 树脂对小麦秸秆水解液中丁醇发酵抑制物的脱除效果[J]. 食品与发酵工业, 2022, 0(2): 26-32
作者姓名:刘国强  易卓林  何开泽  杨林  方扬  赵海  靳艳玲
作者单位:中国科学院成都生物研究所;中国科学院大学生命科学学院;中国科学院环境与应用微生物重点实验室
基金项目:国家重点研发计划项目(2018YFB1501401)。
摘    要:酸水解产生的副产物会严重抑制小麦秸秆水解液丁醇发酵.该文针对树脂对小麦秸秆水解液中丁醇发酵抑制物的脱除效果进行研究.通过强酸性苯乙烯系阳离子交换树脂732及大孔树脂XAD-4对小麦秸秆水解液进行抑制物脱除处理,以未处理组作为对照.离子交换树脂732处理后丁醇得率及含量均高于大孔树脂XAD-4处理.离子交换树脂732处理...

关 键 词:小麦秸秆  丙酮丁醇梭菌  丁醇  树脂  发酵抑制物

Removal effect of resins on butanol fermentation inhibitors in wheat straw hydrolysate
LIU Guoqiang,YI Zhuolin,HE Kaize,YANG Lin,FANG Yang,ZHAO Hai,JIN Yanling. Removal effect of resins on butanol fermentation inhibitors in wheat straw hydrolysate[J]. Food and Fermentation Industries, 2022, 0(2): 26-32
Authors:LIU Guoqiang  YI Zhuolin  HE Kaize  YANG Lin  FANG Yang  ZHAO Hai  JIN Yanling
Affiliation:(Chengdu Institute of Biology,Chinese Academy of Science,Chengdu 610041,China;College of Life Sciences,University of Chinese Academy of Science,Beijing 100049,China;Key Laboratory of Environmental and Applied Microbiology,Chinese Academy of Science,Chengdu 610041,China)
Abstract:By-products from acid hydrolysis of wheat straw can seriously inhibit butanol fermentation. The removal effect of resins on butanol fermentation inhibitors was studied here. Strong acid styrene cation exchange resin 732 and microporous resin XAD-4 were used for the removal of inhibitors in wheat straw hydrolysate, while the untreated group was set as control. Higher yield and concentration of butanol were obtained from the fermentation with resin 732 treatment than those from macroporous resin XAD-4 treatment. Additionally, after resin 732 treatment, fermentation was conducted at a sugar content of 33.23 g/L, and the concentration of butanol and total solvent reached 7.58 and 13.09 g/L, with the yield of 0.23 and 0.40 g/g, which were 5.3 and 5.9 times higher than those from the control group, respectively. In the control group, the accumulation rates of ATP and NADH were slow at the early fermentation stage and reached top values at 24 h. More importantly, during the fermentation, the highest activities of ethanol dehydrogenase and butanol dehydrogenase only reached 29% and 44% of the initial value, while in resin 732 treated group, these could reach as high as 171% and 142%, respectively.
Keywords:wheat straw  Clostridium acetobutylicum  butanol  resin  fermentation inhibitor
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