液液微萃取结合气相色谱-串联质谱测定饮料酒中挥发性酚类化合物

挥发性酚类化合物对饮料酒的风味具有重要影响，同时还具有一定的生理活性。将液液微萃取法与气相色谱-串联质谱联用技术相结合，建立饮料酒中挥发性酚类化合物的测定方法。将酒样稀释至体积分数为10%,再用乙酸乙酯对目标化合物进行萃取，采用气相色谱-串联质谱在动态多反应监测模式下测定挥发性酚类化合物。该方法可准确测定饮料酒中10种挥发性酚类化合物，且线性关系良好(r²≥0.998),检出限及定量限分别为1.0～10.0μg/L和3.5～35.0μg/L;在高、中、低3个浓度水平下的加标回收率为92.3%～100.7%,相对标准偏差为0.3%～3.7%,通过对比试验发现，该方法的准确度与重复性高于顶空固相微萃取-气相色谱质谱法。该方法具有准确、重复性好、有机溶剂用量少等优点，适用于测定不同类型饮料酒中挥发性酚类化合物，研究同时发现挥发性酚类化合物在不同类型饮料酒中的分布存在显著差异。

Analysis of phenols in alcoholic beverages by liquid-liquid microextraction coupled with gas chromatography-tandem mass spectrometry

Phenols have an important influence on the flavor of alcoholic beverages and also have certain physiological activities.The aim of this study was to establish a rapid determination method for the volatile phenolic compounds in alcoholic beverages by liquid-liquid microextraction with gas chromatography-tandem mass spectrometry.The samples were diluted to 10%by volume,then liquid-liquid microextraction was performed with ethyl acetate,and the organic phase was analyzed by gas chromatography-tandem mass spectrometry in a dynamic multiple reaction monitoring mode.The results showed that this method can rapidly quantify 10 Phenols of alcoholic beverages with good linearity(r;≥0.998).The limits of detection and limits of quantification were 1.0-10.0μg/L and 3.5-35.0μg/L respectively.The spiked recoveries were 92.3%-100.7%at high,medium,and low concentration levels with the relative standard deviations(RSD)of 0.3%-3.7%.And there is a significantly different in types and contents of phenols in different types of alcoholic beverages.This method is accurate,only need low amount of organic solvent and is suitable for the quantitative analysis of volatile phenolic compounds in different types of alcoholic beverages.