嗜黏蛋白阿克曼氏菌ATCC BAA-835肠道益生作用的体外评价

嗜黏蛋白阿克曼氏菌(Akkermansia muciniphila,Akk)具有良好的肠道益生作用,与宿主肠道健康、代谢、神经等生理状态密切相关.研究采用体外厌氧培养的方法评价1株Akk模式菌株(ATCC BAA-835)的肠道益生作用,从菌株水平考查菌株对模拟胃肠环境的耐受性、对Caco-2细胞的黏附性,以及利用低聚...

Intestinal probiotic effect of Akkermansia muciniphila ATCC BAA-835 in vitro

Akkermansia muciniphila(Akk) possesses superior intestinal probiotic effect which is closely related to the host′s physiological conditions, including intestinal, metabolic and neurological health. In this study, the intestinal probiotic effect of an Akk model strain(ATCC BAA-835) was evaluated using anaerobic cultivation method in vitro. The probiotic abilities of Akk were investigated, including the tolerance to simulated gastrointestinal environment, adhesion to Caco-2 cells, production of short-chain fatty acids and formation of biofilms utilizing oligosaccharides. The results showed that this Akk stain performed good tolerance to artificial gastrointestinal fluids and bile salts. It could adhere to the microvilli tip of differentiated Caco-2 cells. This Akk strain grew well in the medium with various oligosaccharides. It could quickly consume reducing sugars within 12 h and produce short-chain fatty acids, of which the output of acetic acid was the highest, followed by butyric acid. Akk possessed weak biofilm-forming ability, which could be improved significantly with the addition of oligosaccharides. And Akk could form biofilms at a medium level when the concentration of chito-oligosaccharides was 250 μg/mL. This study could provide further understanding for the microbiological basis of Akk′s intestinal probiotic effect with assessments in vitro including pure microbial culture and cell models.