Lipoxygenase-oxidized soap stock as source of hydroxy conjugated octadecadienoic acids |
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Authors: | E M Emken H J Dutton |
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Affiliation: | (1) Northern Regional Research Laboratory, 61604 Peoria, Illinois |
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Abstract: | A laboratory procedure was developed for the production of hydroxy conjugated octadecadienoic acids (HOCD). Lipoxygenase oxidation
of distilled or crude soap stock produces hydroperoxy conjugated octadecadienoic acids, which can be reduced to HOCD. Crude
aqueous soy flour extracts and defatted soy flour are convenient, effective and inexpensive lipoxygenase sources. This new
procedure allows high substrate concentrations of 100 mg/ml to be used while achieving 75% to 90% oxidation of the available
linoleic acid during a reaction time of 20 to 40 min. Aqueous ethanol and dimethyl sulfoxide (DMSO) solvent systems were compared.
The DMSO system produced higher yields of hydroperoxy acids with low enzyme concentrations. Hydroperoxide yields are good
when short reaction times, high pH levels and free radical scavengers are used.
Presented at the AOCS Meeting, Chicago, September 1970.
No. Utiliz. Res. Dev. Div., ARS, USDA. |
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