海湾扇贝酶解产物清除自由基活性的研究

利用双酶(胰酶0.3%和枯草杆菌中性蛋白酶0.8%)。在50℃、pH值7.0～8.0、料水比1:3的条件下,对海湾扇贝肉酶解4h后制备得到产物.该酶解产物对羟自由基的清除活性IC_(50)为2.01 mg/mL,对超氧阴离子的清除活性IC_(50)为9.54 mg/mL,对DPPH自由基的清除活性IC_(50)为5.90 mg/mL。Sephadex G-15(1.6 cm×68 cm)凝胶色谱结果表明,该酶解产物中分子质量分布在1450～288 u,肽链长度在13.2～2.6的组分具有较强的清除羟自由基和DPPH自由基活性。

Study on Free Radical Scavenging Activity of Enzymatic Hydrolysate of Ar-gopecten irradians Edible Part

Hydrolysate with free radical scavenging activity was prepared from the edible part of Aγ- gopecten irradians by pancreatin(0.3%)and neutral protease(0.8%),under the condition of 50℃,pH7.08～8.0,solid water ratio 1:3 and reaction time 4h.The hydrolysate showed an IC_(50)of 2.01mg/mL for hy- droxyl radical,an IC_(50)of 9.54mg/mL for superoxide radical and an IC_(50)of 5.90mg/mLfor DPPH radical. The hydrolysate was fractionated with a Sephadex G—15 column(1.6cm×68cm).The absorbance of each fractionated tube was measured at 280nm and its molecular weight distribution was calculated according to a regressive equation between the elution volume and the logarithm of the molecular weight.Gel filtration of the hydrolysate on Sephadex G—15 chromatogram yielded five absorbance peaks.Peak C exerted the stron- gest hydroxyl radical and superoxide radical scavenging activity,the molecular weight ranged from 1 450 u to 288 u,and the lengths of peptide linkage located between13.2～2.6.