| 紫外线与氯化锂复合诱变选育L-组氨酸产生菌 |
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| 引用本文： | 邱雁临,梁亮,汪亮.紫外线与氯化锂复合诱变选育L-组氨酸产生菌[J].现代食品科技,2008,24(3):217-219. |
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| 作者姓名： | 邱雁临 梁亮 汪亮 |
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| 作者单位： | 工业微生物湖北省重点实验室,湖北工业大学生物工程学院,湖北,武汉,430068 |
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| 基金项目： | 工业微生物湖北省重点实验室开放基金 |
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| 摘 要： | 以一株谷氨酸棒杆菌(Corynebacterium glutamicum)S6为出发菌株,利用氯化锂、紫外线进行诱变,通过实验证明氯化锂诱变剂量在1.2%时致死率达到82.4%,紫外线在照射30 s时致死率达到81.8%.利用氯化锂诱变谷氨酸棒杆菌S6,所得菌株D3的L-组氨酸产量为243 mg/L,比出发菌株提高10.5%;以紫外线做诱变S6,所得高产菌株U1产量256 mg/L,比出发菌株提高8.5%;以紫外线、氯化锂复合诱变S6,所得菌株N1产量为251 mg/L,比出发菌株提高13.6%,结果显示,经紫外线与氯化锂复合诱变后的菌株N1产L-组氨酸的产量最高,比氯化锂诱变后的菌株产L-组氨酸量提高3.1%,比紫外线诱变后的菌株产L-组氨酸量提高5.1%.
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| 关 键 词： | 紫外线 氯化锂 复合诱变 L-组氨酸 |
| 文章编号： | 1673-9078(2008)03-0217-03 |
| 收稿时间： | 2007/11/15 0:00:00 |
| 修稿时间： | 2007年11月15 |
Mutation Screening of L-histidine Producing Strain by UV and LiCl |
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| QIU Yan-lin,LIANG Liang,WANG Liang.Mutation Screening of L-histidine Producing Strain by UV and LiCl[J].Modern Food Science & Technology,2008,24(3):217-219. |
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| Authors: | QIU Yan-lin LIANG Liang WANG Liang |
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| Affiliation: | (The province key laboratory of Industrial Microorganism, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China) |
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| Abstract: | A L-histidine producing strain was mutated and screened from Corynebacterium glutamicum S6 by LiCl and UV. Results showed that the UV-induced lethality was 81.8% with the irradiating time of 30s, while the LiCl-induced lethality was 82.4% with the LiCl concentration of 1.2%. Using three mutants, D3 by LiCl, U1 by UV, and N1 multiple mutated by UV and LiCl, the L-histidine yields reached 256 mg/L, 243 mg/L and 251 mg/L, respectively, which were 8.5%, 10.5% , and 13.6% respectively higher than that by the original strain S6. |
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| Keywords: | UV LiCl Composite mutation L-histidine |
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