| 抗咽喉炎常见菌IgY抗体的制备及其特性的研究 |
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| 引用本文： | 张玲,董涛,宋立兵.抗咽喉炎常见菌IgY抗体的制备及其特性的研究[J].粉末涂料与涂装,2006,19(2):191-193. |
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| 作者姓名： | 张玲 董涛 宋立兵 |
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| 作者单位： | 华南肿瘤学国家重点实验室 中山大学肿瘤防治中心实验研究部，中山大学中山医学院免疫学教研室，华南肿瘤学国家重点实验室，中山大学肿瘤防治中心实验研究部，广州510060，广州510089，广州510060 |
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| 摘 要： | 目的制备抗β-溶血性链球菌、G簇溶血性链球菌、金黄色葡萄球菌3种咽喉炎常见菌IgY抗体,并分析其特性。方法采用超声波破碎法将3种咽喉菌制备成可溶性抗原液,免疫产蛋母鸡,从鸡蛋黄中提取IgY抗体,应用免疫电泳、ELISA、SDS-PAGE检测抗3种咽喉菌IgY抗体的特性及热稳定性。结果抗原免疫母鸡10d后,鸡蛋黄中即有抗3种咽喉菌的IgY抗体产生,60d时抗体滴度高达1∶102400,IgY抗体滴度在室温可保持5~6个月不变。结论制备的抗3种咽喉菌IgY抗体滴度高,特异性强,稳定性好。
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| 关 键 词： | 咽炎 IgY 特性 |
| 收稿时间： | 2005-06-16 |
| 修稿时间： | 2005年6月16日 |
Preparation and Characteristics of Antibody against Common Bacteria Causing Sore Throat |
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| ZHANG Ling,DONG Tao,SONG Li-bing.Preparation and Characteristics of Antibody against Common Bacteria Causing Sore Throat[J].Chinese Journal of Biologicals,2006,19(2):191-193. |
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| Authors: | ZHANG Ling DONG Tao SONG Li-bing |
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| Affiliation: | State Key Laboratory of Oncology in Southern China ,Department of Experimental Research, Cancer Center, Sun Yat-sen University, Guangzhou 510060, China |
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| Abstract: | Objective To prepare the antibodies against three kinds of common bacteria causing sore throat,i.e.β-streptococcus hemolyticus,streptococcus hemolyticus group G and staphylococcus aureus,and anlayze their properties.Methods Prepare the three kinds of bacteria into soluble antigens and immunize to hens.Extract IgY antibodies from the yolk,analyze their property by immunoeletrophoresis,ELISA and SDS-PAGE and study their thermostability.Results The IgY against three kinds of bacteria were isolated from the yolk only 10 d after immunization.Sixty days after immunization,the IgY titer reached 1∶102 400 and showed no significant change 5-6 months after storage at room temperature.Conclusion The prepared IgY antibodies showed high titers,specificities and stabilities. |
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| Keywords: | Sore throat IgY Characteristics |
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