乳酸乳球菌乳酸亚种高产胆盐水解酶发酵条件的优化研究

本文利用从藏灵菇中筛选的产胆盐水解酶的乳酸乳球菌乳酸亚种(Lactococcuslactissubsp.lactis)研究提高酶活力的环境因素。针对主要影响胆盐水解酶合成的四个因素,采用四因素三水平L9(34)正交试验确定了高产胆盐水解酶优化发酵条件:葡萄糖添加量为3%、大豆蛋白胨添加量为2%、发酵温度为37℃、接种量为2%。在优化条件下,胆盐水解酶活力是优化前的11.84倍。

Studies on Optimization of Fermentation Conditions of Lactococcus lactis subsp.lactis Bile Salt Hydrolase

In this paper,we use the Lactococcus lactis subsp.lactis of bile salt hydrolase screened from kefir grains to the study environment factors of increasing enzyme vires. For four factors to affect synthesize of bile salt hydrolase,the fermentation conditions of high yield bile salt hydrolase were determined by the four factors and three levels orthogonal test L9(34). As followglucose 2%,soya-peptone 2%,fermentation temperature 37℃,amount of inoculation 2%. In optimization conditions,the vires of bile salt hydrolase is 11.84 times before optimize.