Facile direct electron transfer in glucose oxidase modified electrodes |
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Authors: | Dan Wang |
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Affiliation: | a Department of Chemistry and Biochemistry, Ohio University, Athens, OH 45701, USA b Suzhou Institute of Nano Tech and Nano Bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Suzhou Industrial Park, Suzhou, Jiangsu 215125, PR China |
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Abstract: | Glucose oxidase (GOx) is widely used in the glucose biosensor industry. However, mediatorless direct electron transfer (DET) from GOx to electrode surfaces is very slow. Recently, mediatorless DET has been reported via the incorporation of nanomaterials such as carbon nanotubes and nanoparticles in the modification of electrodes. Here we report GOx electrodes showing DET without the need for any nanomaterials. The enzyme after immobilization with poly-l-lysine (PLL) and Nafion® retains the biocatalytic activities and oxidizes glucose efficiently. The amperometric response of Nafion-PLL-GOx modified electrode is linearly proportional to the concentration of glucose up to 10 mM with a sensitivity of 0.75 μA/mM at a low detection potential (−0.460 V vs. Ag/AgCl). The methodology developed in this study will have impact on glucose biosensors and biofuel cells and may potentially simplify enzyme immobilization in other biosensing systems. |
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Keywords: | Glucose oxidase Direct electron transfer l-lysine" target="_blank">Poly-l-lysine Nafion Glucose biosensor |
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