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A peptide cross-linked polyacrylamide hydrogel for the detection of human neutrophil elastase |
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| Authors: | Dzaraini Kamarun Lilia Milanesi Steffi Krause |
| Affiliation: | a Department of Polymer Technology, Faculty of Applied Science, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia b Department of Chemistry, University of Sheffield, Sheffield S3 7HF, UK c School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, E1 4NS London, UK |
| Abstract: | Polyacrylamide hydrogel films cross-linked with the peptide sequence AAPVAAK were synthesised on piezoelectric quartz crystals. Degradation of the hydrogel films was monitored using a combination of quartz crystal microbalance (QCM) and electrochemical impedance measurements. The films were shown to degrade in the presence of human neutrophil elastase (HNE). The rate of degradation was directly related to the enzyme activity making the system suitable for the detection of HNE in the activity range from 0.72 to 30 U mL−1. The films were not affected by non-specific adsorption. Film degradation was only accompanied by changes in the QCM signal, while no significant impedance change was observed during degradation. |
| Keywords: | Peptide cross-linked hydrogel Polyacrylamide Enzymatic degradation Human neutrophil elastase Quartz crystal microbalance Impedance |
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